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Magnetic microparticle concentration and collection using a mechatronic magnetic ratcheting system
- Source :
- PLoS ONE, Vol 16, Iss 2, p e0246124 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Magnetic ratcheting cytometry is a promising approach to separate magnetically-labeled cells and magnetic particles based on the quantity of magnetic material. We have previously reported on the ability of this technique to separate magnetically-labeled cells. Here, with a new chip design, containing high aspect ratio permalloy micropillar arrays, we demonstrate the ability of this technique to rapidly concentrate and collect superparamagnetic iron oxide particles. The platform consists of a mechatronic wheel used to generate and control a cycling external magnetic field that impinges on a “ratcheting chip.” The ratcheting chip is created by electroplating a 2D array of high aspect ratio permalloy micropillars onto a glass slide, which is embedded in a thin polymer layer to create a planar surface above the micropillars. By varying magnetic field frequency and direction through wheel rotation rate and angle, we direct particle movement on chip. We explore the operating conditions for this system, identifying the effects of varying ratcheting frequency, along with time, on the dynamics and resulting concentration of these magnetic particles. We also demonstrate the ability of the system to rapidly direct the movement of superparamagnetic iron oxide particles of varying sizes. Using this technique, 2.8 μm, 500 nm, and 100 nm diameter superparamagnetic iron oxide particles, suspended within an aqueous fluid, were concentrated. We further define the ability of the system to concentrate 2.8 μm superparamagnetic iron oxide particles, present in a liquid suspension, into a small chip surface area footprint, achieving a 100-fold surface area concentration, and achieving a concentration factor greater than 200%. The achieved concentration factor of greater than 200% could be greatly increased by reducing the amount of liquid extracted at the chip outlet, which would increase the ability of achieving highly sensitive downstream analytical techniques. Magnetic ratcheting-based enrichment may be useful in isolating and concentrating subsets of magnetically-labeled cells for diagnostic automation.
- Subjects :
- Microfluidics
Cell Separation
Spectrum Analysis Techniques
Materials
Fluorescence-Activated Cell Sorting
Multidisciplinary
Physics
Magnetism
Oxides
Microfluidic Analytical Techniques
Condensed Matter Physics
Chip
Magnetic field
Chemistry
Aspect Ratio
Spectrophotometry
Physical Sciences
Magnets
Microtechnology
Engineering and Technology
Optoelectronics
Medicine
Cytophotometry
Fluidics
Research Article
Superparamagnetism
Permalloy
Materials science
Science
Materials Science
Geometry
Research and Analysis Methods
Iron Oxides
Suspensions
Humans
Particle Size
business.industry
Magnetic Phenomena
Chemical Compounds
equipment and supplies
Magnetic Fields
Tissue Array Analysis
Mixtures
Magnet
Particle
Magnetic nanoparticles
business
human activities
Mathematics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....252f74d38226f77fd692fb4a5b9dff23