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Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes
- Source :
- AJP-Endocrinology and Metabolism, AJP-Endocrinology and Metabolism, American Physiological Society, 2006, 291, pp.E372-E380. ⟨10.1152/ajpendo.00482.2005⟩, American Journal of Physiology. Endocrinology and Metabolism (291), E372-E380. (2006)
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- The function of adipocytes interspersed between myofiber fasciculi in skeletal muscle physiology and physiopathology is poorly documented. Because regional differences in adipocyte features have been reported in various species, we hypothesized that lipid metabolism and secretory function of intramuscular (IM) adipocytes differ from that of nonmuscular adipocytes. In the present study, adipocytes isolated from trapezius muscle were compared with subcutaneous and perirenal adipocytes in growing pigs. Between 80 and 210 days of age, gene expressions and/or activities of enzymes involved in lipogenesis or lipolysis were much lower ( P < 0.05) in adipocytes isolated from muscle than in those from other locations. Insulin-induced lipogenesis and lipolytic efficiency after catecholamine addition were also the lowest ( P < 0.05) in IM adipocytes. In these cells, the age-related increase (+300%) in the ratio of mRNA levels of fatty acid synthase to hormone-sensitive lipase paralleled the enlargement of adipocyte diameters (+70%, P < 0.05) and the increase in lipid content in muscle (+135%, P < 0.05) during growth. Expressions of genes coding for leptin, adiponectin, and IGF-I, as well as for various hormonal receptors, were lower ( P < 0.05) in IM adipocytes than in other adipocytes, whereas levels of TNF-α mRNA did not differ between sites. Interestingly, IGF-II mRNA levels were higher ( P < 0.05) in IM adipocytes than in other adipocytes. These data support the view that IM fat is not just an ectopic extension of other fat locations but displays specific biological features during growth.
- Subjects :
- medicine.medical_specialty
expression génique
Physiology
muscle
Swine
Endocrinology, Diabetes and Metabolism
Biology
adipocyte
Kidney
03 medical and health sciences
chemistry.chemical_compound
Physiology (medical)
Adipocyte
Internal medicine
medicine
Adipocytes
Lipolysis
Myocyte
Animals
Muscle, Skeletal
lipide
Cells, Cultured
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Skin
0303 health sciences
Endocrinology and metabolism
métabolisme lipidique
0402 animal and dairy science
Skeletal muscle
Lipid metabolism
muscle squelettique
04 agricultural and veterinary sciences
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Lipid Metabolism
040201 dairy & animal science
Pathophysiology
lipide intramusculaire
Endocrinology
medicine.anatomical_structure
chemistry
Lipogenesis
Endocrinologie et métabolisme
Female
Function (biology)
Subjects
Details
- Language :
- English
- ISSN :
- 01931849 and 15221555
- Database :
- OpenAIRE
- Journal :
- AJP-Endocrinology and Metabolism, AJP-Endocrinology and Metabolism, American Physiological Society, 2006, 291, pp.E372-E380. ⟨10.1152/ajpendo.00482.2005⟩, American Journal of Physiology. Endocrinology and Metabolism (291), E372-E380. (2006)
- Accession number :
- edsair.doi.dedup.....2560672cebccc14e72c89cf484ffe72e