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A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain
- Source :
- Iranian Journal of Microbiology, Iranian Journal of Microbiology, Vol 12, Iss 1 (2020)
- Publication Year :
- 2020
- Publisher :
- Tehran University of Medical Sciences, 2020.
-
Abstract
- Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Pre- ceding researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis. Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs. Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay. Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.
- Subjects :
- Microbiology (medical)
Bordetella pertussis
lcsh:QR1-502
Filamentous haemagglutinin adhesin
Extraction
Pertussis toxin
01 natural sciences
Microbiology
lcsh:Microbiology
Western blot
0502 economics and business
medicine
0101 mathematics
biology
medicine.diagnostic_test
Chemistry
Outer membrane vesicle
010102 general mathematics
05 social sciences
biology.organism_classification
Blot
Pertussis vaccine
Original Article
Pertactin
Bacterial outer membrane
Vaccine
050203 business & management
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20084447 and 20083289
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Iranian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....256479675187612c56cfde79b17ab188