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The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor α with the small GTPase Rac
- Source :
- BMC Biochemistry, BMC Biochemistry, BioMed Central, 2010, 11 (1), pp.48. ⟨10.1186/1471-2091-11-48⟩, BMC Biochemistry, Vol 11, Iss 1, p 48 (2010), BMC Biochemistry, 2010, 11 (1), pp.48. ⟨10.1186/1471-2091-11-48⟩
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- Background RhoGDI proteins are important regulators of the small GTPase Rac, because they shuttle Rac from the cytoplasm to membranes and also protect Rac from activation, deactivation and degradation. How the binding and release of Rac from RhoGDI is regulated is not precisely understood. Results We report that the non-receptor tyrosine kinase Fer is able to phosphorylate RhoGDIα and form a direct protein complex with it. This interaction is mediated by the C-terminal end of RhoGDIα. Activation of Fer by reactive oxygen species caused increased phosphorylation of RhoGDIα and pervanadate treatment further augmented this. Tyrosine phosphorylation of RhoGDIα by Fer prevented subsequent binding of Rac to RhoGDIα, but once a RhoGDIα-Rac complex was formed, the Fer kinase was not able to cause Rac release through tyrosine phosphorylation of preformed RhoGDIα-Rac complexes. Conclusions These results identify tyrosine phosphorylation of RhoGDIα by Fer as a mechanism to regulate binding of RhoGDIα to Rac.
- Subjects :
- lcsh:Animal biochemistry
MESH: rac GTP-Binding Proteins
Plasma protein binding
Biochemistry
Substrate Specificity
MESH: Tyrosine
chemistry.chemical_compound
Chlorocebus aethiops
MESH: Guanine Nucleotide Dissociation Inhibitors
lcsh:QD415-436
Small GTPase
MESH: Animals
Phosphorylation
Tyrosine
[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
Guanine Nucleotide Dissociation Inhibitors
0303 health sciences
Kinase
030302 biochemistry & molecular biology
Protein-Tyrosine Kinases
rac GTP-Binding Proteins
Cell biology
Rac GTP-Binding Proteins
MESH: COS Cells
COS Cells
Tyrosine kinase
Research Article
Protein Binding
MESH: Cell Line, Tumor
MESH: Rats
Biology
MESH: Protein-Tyrosine Kinases
lcsh:Biochemistry
03 medical and health sciences
Cell Line, Tumor
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Animals
Humans
rho-Specific Guanine Nucleotide Dissociation Inhibitors
MESH: Protein Binding
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
lcsh:QP501-801
Molecular Biology
030304 developmental biology
rho Guanine Nucleotide Dissociation Inhibitor alpha
MESH: Humans
MESH: Phosphorylation
Tyrosine phosphorylation
MESH: Cercopithecus aethiops
Rats
chemistry
MESH: Substrate Specificity
Subjects
Details
- Language :
- English
- ISSN :
- 14712091
- Database :
- OpenAIRE
- Journal :
- BMC Biochemistry, BMC Biochemistry, BioMed Central, 2010, 11 (1), pp.48. ⟨10.1186/1471-2091-11-48⟩, BMC Biochemistry, Vol 11, Iss 1, p 48 (2010), BMC Biochemistry, 2010, 11 (1), pp.48. ⟨10.1186/1471-2091-11-48⟩
- Accession number :
- edsair.doi.dedup.....2589ab5d00f475aeaca772ea28c1ef1a