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Two-color far-field fluorescence nanoscopy
- Source :
- Biophysical Journal
- Publication Year :
- 2007
-
Abstract
- We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The stimulated emission depletion beams deliver a lateral resolution of
- Subjects :
- Materials science
Biophysical Letters
Biophysics
Analytical chemistry
Receptors, Cytoplasmic and Nuclear
Receptors, Cell Surface
Endosomes
PC12 Cells
Sensitivity and Specificity
Nanoclusters
Mitochondrial Proteins
Mitochondrial Precursor Protein Import Complex Proteins
Fluorescence microscope
Animals
Nanotechnology
Stimulated emission
Emission spectrum
Image resolution
Microscopy, Confocal
Resolution (electron density)
Membrane Transport Proteins
Reproducibility of Results
Colocalization
Image Enhancement
Fluorescence
Rats
Proton-Translocating ATPases
Microscopy, Fluorescence, Multiphoton
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Biophysical Journal
- Accession number :
- edsair.doi.dedup.....258fc8d4fd6fdc14f6d087cdf0c4a880