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Overexpression of KIAA0101 Promotes the Progression of Non-small Cell Lung Cancer
- Source :
- Journal of Cancer
- Publication Year :
- 2020
- Publisher :
- Ivyspring International Publisher, 2020.
-
Abstract
- Lung cancer is the leading cause of cancer related death worldwide, with a continue-rising incidence. The proliferating cell nuclear antigen binding protein KIAA0101 is highly expressed in various types of cancer, including non-small cell lung cancer (NSCLC). However, its biological role and underlying mechanisms in NSCLC remains unclear. We downloaded KIAA0101 mRNA and clinical data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), and verified the KIAA0101 expression by conducting experiments of immunochemistry (IHC), immunofluorescence (IF), quantitative real-time PCR (qRT-PCR) and western-blot. Functional experiments were performed to explore the biological roles in vitro and in vivo. The results showed that KIAA0101 was overexpressed in NSCLC tissues and cell lines. High KIAA0101 expression was associated with high T stage, nodal invasion, advanced tumor stage, and poor overall survival (P
- Subjects :
- 0301 basic medicine
diagnosis
proliferation
Immunofluorescence
03 medical and health sciences
0302 clinical medicine
In vivo
Immunochemistry
medicine
Lung cancer
non-small cell lung cancer
Gene knockdown
biology
medicine.diagnostic_test
KIAA0101
medicine.disease
Proliferating cell nuclear antigen
030104 developmental biology
Oncology
Cell culture
030220 oncology & carcinogenesis
biology.protein
Cancer research
Immunohistochemistry
prognosis
Research Paper
Subjects
Details
- ISSN :
- 18379664
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of Cancer
- Accession number :
- edsair.doi.dedup.....25a6956401c272aa96566e554a999411