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Understanding the mechanism of Chikungunya virus vector competence in three species of mosquitoes
- Source :
- Medical and Veterinary Entomology. 33:375-387
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Chikungunya virus (CHIKV) is primarily transmitted by Aedes spp. mosquitoes. The present study investigated vector competence for CHIKV in Aedes aegypti and Aedes albopictus mosquitoes found in Madurai, South India. The role of receptor proteins on midguts contributing to permissiveness of CHIKV to Aedes spp. mosquitoes was also undertaken. Mosquitoes were orally infected with CHIKV DRDE-06. Infection of midguts and dissemination to heads was confirmed by immunofluorescence assay at different time points. A plaque assay was performed from mosquito homogenates at different time points to study CHIKV replication. Presence of putative CHIKV receptor proteins on mosquito midgut epithelial cells was detected by virus overlay protein binding assay (VOPBA). The identity of these proteins was established using mass spectrometry. CHIKV infection of Ae. aegypti and Ae. albopictus midguts and dissemination to heads was observed to be similar. A plaque assay performed with infected mosquito homogenates revealed that CHIKV replication dynamics was similar in Aedes sp. mosquitoes until 28 days post infection. VOPBA performed with mosquito midgut membrane proteins revealed that prohibitin could serve as a putative CHIKV receptor on Aedes mosquito midguts, whereas an absence of CHIKV binding protein/s on Culex quinquefasciatus midguts can partially explain the non-permissiveness of these mosquitoes to infection.
- Subjects :
- 0301 basic medicine
Aedes albopictus
education
030231 tropical medicine
India
Mosquito Vectors
Aedes aegypti
Biology
medicine.disease_cause
Virus
03 medical and health sciences
0302 clinical medicine
Aedes
parasitic diseases
medicine
Animals
Chikungunya
Ecology, Evolution, Behavior and Systematics
Virus quantification
General Veterinary
fungi
virus diseases
Midgut
030108 mycology & parasitology
biology.organism_classification
Virology
Culex quinquefasciatus
Culex
Insect Science
Vector (epidemiology)
Chikungunya Fever
Parasitology
Chikungunya virus
Subjects
Details
- ISSN :
- 13652915 and 0269283X
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Medical and Veterinary Entomology
- Accession number :
- edsair.doi.dedup.....25a9c31c587c195d0ca5c647d5b2d205