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Stable expression of Rice dwarf virus Pns10 suppresses the post-transcriptional gene silencing in transgenic Nicotiana benthamiana plants
- Source :
- Acta Virologica. 54:99-104
- Publication Year :
- 2010
- Publisher :
- Frontiers Media SA, 2010.
-
Abstract
- UNLABELLED RNA silencing is a conserved mechanism that defends against viral infection and retrotransposon activity for protection of the genome. Segment 10 (S10) of Rice dwarf virus (RDV) encodes Pns10 protein, a viral suppressor of RNAi that suppresses the host RNA silencing machinery. In this study, we obtained stable transgenic RDV-S10 Nicotiana benthamiana plants expressing Pns10. Suppression of post-transcriptional gene silencing (PTGS) by Pns10 supported the conclusion that this protein exhibited the RNA silencing suppressor activity. In particular, the transgenic plants stably expressing a viral suppressor of RNAi (VSR) provide a model system for investigating the mechanism of RNA silencing. KEYWORDS RNA silencing; VSR; Rice dwarf virus; Pns10; transgenic plant.
- Subjects :
- Genes, Viral
RNA-induced silencing complex
viruses
Green Fluorescent Proteins
Trans-acting siRNA
Gene Expression
Nicotiana benthamiana
Biology
Reoviridae
RNA interference
Virology
Tobacco
Gene expression
Gene silencing
fungi
food and beverages
Oryza
General Medicine
Plants, Genetically Modified
biology.organism_classification
Recombinant Proteins
Cell biology
RNA silencing
Infectious Diseases
Rice dwarf virus
RNA Interference
Subjects
Details
- ISSN :
- 0001723X
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Acta Virologica
- Accession number :
- edsair.doi.dedup.....25aa46d57c7de42a68259fb35bc81b2f