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Immunoglobulin profiling identifies unique signatures in patients with Kawasaki disease during intravenous immunoglobulin treatment
- Source :
- Human Molecular Genetics
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Identifying the causes of high fever syndromes such as Kawasaki disease (KD) remains challenging. To investigate pathogen exposure signatures in suspected pathogen-mediated diseases such as KD, we performed immunoglobulin (Ig) profiling using a next-generation sequencing method. After intravenous Ig (IVIG) treatment, we observed disappearance of clonally expanded IgM clonotypes, which were dominantly observed in acute-phase patients. The complementary-determining region 3 (CDR3) sequences of dominant IgM clonotypes in acute-phase patients were commonly observed in other Ig isotypes. In acute-phase KD patients, we identified 32 unique IgM CDR3 clonotypes shared in three or more cases. Furthermore, before the IVIG treatment, the sums of dominant IgM clonotypes in IVIG-resistant KD patients were significantly higher than those of IVIG-sensitive KD patients. Collectively, we demonstrate a novel approach for identifying certain Ig clonotypes for potentially interacting with pathogens involved in KD; this approach could be applied for a wide variety of fever-causing diseases of unknown origin.
- Subjects :
- 0301 basic medicine
Fever
Treatment outcome
Mucocutaneous Lymph Node Syndrome
03 medical and health sciences
0302 clinical medicine
hemic and lymphatic diseases
Genetics
medicine
Humans
In patient
Molecular Biology
Pathogen
Genetics (clinical)
biology
Immunoglobulins, Intravenous
Articles
General Medicine
medicine.disease
High fever
Immunoglobulin Isotypes
Treatment Outcome
030104 developmental biology
Immunoglobulin M
Intravenous IG
Immunology
biology.protein
Kawasaki disease
Antibody
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14602083 and 09646906
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Human Molecular Genetics
- Accession number :
- edsair.doi.dedup.....25cd4481f2a38529922255949dcf6ec6