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Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging
- Source :
- Science China. Life sciences. 63(10)
- Publication Year :
- 2019
-
Abstract
- Despite the wide application of super-resolution (SR) microscopy in biological studies of cells, the technology is rarely used to monitor functional changes in live cells. By combining fast spinning disc-confocal structured illumination microscopy (SD-SIM) with loading of cytosolic fluorescent Ca2+ indicators, we have developed an SR method for visualization of regional Ca2+ dynamics and related cellular organelle morphology and dynamics, termed SR calcium lantern imaging. In COS-7 cells stimulated with ATP, we have identified various calcium macrodomains characterized by different types of Ca2+ release from endoplasmic reticulum (ER) stores. Finally, we demonstrated various roles of mitochondria in mediating calcium signals from different sources; while mitochondria can globally potentiate the Ca2+ entry associated with store release, mitochondria also locally control Ca2+ release from the neighboring ER stores and assist in their refilling processes.
- Subjects :
- Endoplasmic reticulum
Optical Imaging
chemistry.chemical_element
Calcium
Mitochondrion
Endoplasmic Reticulum
Fluorescence
Superresolution
General Biochemistry, Genetics and Molecular Biology
Mitochondria
Cytosol
chemistry
Microscopy
Organelle
COS Cells
Chlorocebus aethiops
Biophysics
Animals
Calcium Signaling
General Agricultural and Biological Sciences
General Environmental Science
Fluorescent Dyes
Subjects
Details
- ISSN :
- 18691889
- Volume :
- 63
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Science China. Life sciences
- Accession number :
- edsair.doi.dedup.....25e12bc8ef19142cfb40726b6ccaab48