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Human epidermal langerhans cells express the immunoregulatory enzyme indoleamine 2,3-dioxygenase
- Source :
- The Journal of investigative dermatology. 123(2)
- Publication Year :
- 2004
-
Abstract
- Langerhans cells (LC) are a special subset of dendritic cells integrating cutaneous immunity. The study of LC function is of major interest not only for efforts of vaccine design and immunotherapy but also for gaining an insight into the pathogenesis of immune-mediated cutaneous diseases and neoplasias. Recently, defined antigen-presenting cells were described that express indoleamine 2,3-dioxygenase (IDO) and inhibit T cell proliferation in vitro and in vivo. Here, we show that stimulation with interferon-gamma (IFN-gamma) induces the expression of functionally active IDO in highly purified human epidermal LC. The induction of IDO after stimulation of LC with IFN-gamma seems to follow a defined kinetic with rapid upregulation followed by a downregulation after about 24 h of culture. Accordingly, proliferation of T cells induced by anti-CD3 antibodies was modulated by supernatants of IFN-gamma-activated human epidermal LC. Importantly, downregulation of T cell proliferation by supernatants of 24 h IFN-gamma-activated LC was prevented by inhibition of IDO. These results indicate that LC not only have the capacity to stimulate but also to inhibit T cells, and suggest that LC possess an immunoregulatory function in promoting T cell tolerance by production of IDO.
- Subjects :
- Keratinocytes
Langerhans cell
indoleamine 2,3-dioxygenase
medicine.medical_treatment
T cell
T-Lymphocytes
Antineoplastic Agents
Dermatology
Biology
Biochemistry
Gene Expression Regulation, Enzymologic
Monocytes
Interferon-gamma
Downregulation and upregulation
medicine
Immune Tolerance
Humans
Indoleamine-Pyrrole 2,3,-Dioxygenase
RNA, Messenger
Antigen-presenting cell
Indoleamine 2,3-dioxygenase
Molecular Biology
Cells, Cultured
tolerance
Immunotherapy
Cell Biology
In vitro
Tryptophan Oxygenase
Kinetics
medicine.anatomical_structure
Epidermal Cells
Langerhans Cells
Immunology
biology.protein
Cancer research
Antibody
Epidermis
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 123
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Journal of investigative dermatology
- Accession number :
- edsair.doi.dedup.....260d3b14d6900eb2fba7a69880fb5493