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Effect of ABA-beta-D-glucopyranosyl ester and activity of ABA-beta-D-glucosidase in Arabidopsis thaliana

Authors :
Hisashi Kato-Noguchi
Yukitoshi Tanaka
Source :
Journal of plant physiology. 165(7)
Publication Year :
2007

Abstract

Exogenously applied ABA-beta-D-glucopyranosyl ester (ABA-GE) inhibited hypocotyl growth of Arabidopsis seedlings at concentrations greater than 0.3 micromol/L, and the concentration for 50% inhibition of hypocotyl growth was 1.8 micromol/L. ABA-beta-D-glucosidase activity in Arabidopsis seedlings was 17 nmol/mg protein/mim and increased by exogenously applied ABA-GE. The pH optimum of this enzyme in crude extract of Arabidopsis seedlings was 6.0 for the assay in the ABA-GE to ABA direction and its K(m) value for ABA-GE (pH 6.0) was 0.41 mmol/L. These results suggests that exogenously applied ABA-GE may be absorbed by roots and hydrolyzed by ABA-beta-D-glucosidase and librated free ABA may induce growth inhibition in Arabidopsis hypocotyls.

Details

ISSN :
16181328
Volume :
165
Issue :
7
Database :
OpenAIRE
Journal :
Journal of plant physiology
Accession number :
edsair.doi.dedup.....26249eb289fd89497dd9f4a0d49bc5f8