Supplementary Information from Interleukin-27 Exerts Its Antitumor Effects by Promoting Differentiation of Hematopoietic Stem Cells to M1 Macrophages

This file contains Supplemental Table 1, Supplementary Figure Legends, and Supplementary Figures. Supplemental Table 1 shows primers used in this study. Supplementary Figure Legends describe the legend for each supplementary figure. Figure S1 shows the gating strategy in flow cytometry analysis. Figure S2 shows that IL-27 augments the infiltration of CD11b+ myeloid cells into MC38 tumor. Figure S3 shows that IL-27 promotes the differentiation into M1 macrophages in MC38 tumor-bearing mice. Figure S4 shows that IL-27 much less but significantly augments the infiltration of M1 macrophages into B16F10 tumor when the tumor size in B16F10-IL-27 tumor-bearing mice reaches the similar tumor size to that in control tumor-bearing mice. Figure S5 shows that IL-27 inhibits the differentiation into immunosuppressive macrophages in MC38 tumor-bearing mice. Figure S6 shows the therapeutic potential of the CD11b+ myeloid cells purified from tumors of B16F10-IL-27 tumor-bearing mice on pre-existing tumor model. Figure S7 shows that IL-27-induced MC38 tumor-infiltrating myeloid cells exert direct antitumor effects by killing tumor through NO. Figure S8 shows that the enhanced mRNA expression of immune checkpoint molecules and TGF-β1 in CD11b+ myeloid cells in IL-27-expressing tumor-bearing mice.