Back to Search
Start Over
Fibrinogen-thrombin collagen patch reinforcement of high-risk colonic anastomoses in rats
- Source :
- World Journal of Gastrointestinal Surgery
- Publication Year :
- 2016
- Publisher :
- Baishideng Publishing Group Inc., 2016.
-
Abstract
- Aim To evaluate the effectiveness of human fibrinogen-thrombin collagen patch (TachoSil®) in the reinforcement of high-risk colon anastomoses. Methods A quasi-experimental study was conducted in Wistar rats (n = 56) that all underwent high-risk anastomoses (anastomosis with only two sutures) after colectomies. The rats were divided into two randomized groups: Control group (24 rats) and treatment group (24 rats). In the treatment group, high-risk anastomosis was reinforced with TachoSil® (a piece of TachoSil® was applied over this high-risk anastomosis, covering the gap). Leak incidence, overall survival, intra-abdominal adhesions, and histologic healing of anastomoses were analyzed. Survivors were divided into two subgroups and euthanized at 15 and 30 d after intervention in order to analyze the adhesions and histologic changes. Results Overall survival was 71.4% and 57.14% in the TachoSil® group and control group, respectively (P = 0.29); four rats died from other causes and six rats in the treatment group and 10 in the control group experienced colonic leakage (P > 0.05). The intra-abdominal adhesion score was similar in both groups, with no differences between subgroups. We found non-significant differences in the healing process according to the histologic score used in both groups (P = 0.066). Conclusion In our study, the use of TachoSil® was associated with a non-statistically significant reduction in the rate of leakage in high-risk anastomoses. TachoSil® has been shown to be a safe product because it does not affect the histologic healing process or increase intra-abdominal adhesions.
- Subjects :
- medicine.medical_specialty
Colectomies
biology
Colon
Anastomosis
business.industry
Adhesion (medicine)
TachoSil
Basic Study
030230 surgery
medicine.disease
Fibrin
Rats
Surgery
03 medical and health sciences
TachoSil®
0302 clinical medicine
030220 oncology & carcinogenesis
Overall survival
medicine
biology.protein
Leak
business
Subjects
Details
- ISSN :
- 19489366
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- World Journal of Gastrointestinal Surgery
- Accession number :
- edsair.doi.dedup.....26285ff2e1b8a865a01b9faa539b763f