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Ex vivo generation of mature and functional human smooth muscle cells differentiated from skeletal myoblasts

Authors :
Jean-Marc Zini
Xu-hui Liu
Karine Vauchez
Jean-Pierre Marolleau
Marie-Noëlle Lacassagne
Isabelle Robert
Georges Uzan
Marc Benbunan
Gérard Tobelem
Jérôme Larghero
Sophie Le Ricousse-Roussanne
Jean-Thomas Vilquin
Véronique Barateau
Brigitte Ternaux
Philippe Foubert
Source :
Experimental cell research. 313(7)
Publication Year :
2006

Abstract

We described the ex vivo production of mature and functional human smooth muscle cells (SMCs) derived from skeletal myoblasts. Initially, myoblasts expressed all myogenic cell-related markers such as Myf5, MyoD and Myogenin and differentiate into myotubes. After culture in a medium containing vascular endothelial growth factor (VEGF), these cells were shown to have adopted a differentiated SMC identity as demonstrated by αSMA, SM22α, calponin and smooth muscle-myosin heavy chain expression. Moreover, the cells cultured in the presence of VEGF did not express MyoD anymore and were unable to fuse in multinucleated myotubes. We demonstrated that myoblasts-derived SMCs (MDSMCs) interacted with endothelial cells to form, in vitro , a capillary-like network in three-dimensional collagen culture and, in vivo , a functional vascular structure in a Matrigel implant in nonobese diabetic-severe combined immunodeficient mice. Based on the easily available tissue source and their differentiation into functional SMCs, these data argue that skeletal myoblasts might represent an important tool for SMCs-based cell therapy.

Details

ISSN :
00144827
Volume :
313
Issue :
7
Database :
OpenAIRE
Journal :
Experimental cell research
Accession number :
edsair.doi.dedup.....2667964fdc41cdc08ee7a8df8abe4fbb