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Ex vivo generation of mature and functional human smooth muscle cells differentiated from skeletal myoblasts
- Source :
- Experimental cell research. 313(7)
- Publication Year :
- 2006
-
Abstract
- We described the ex vivo production of mature and functional human smooth muscle cells (SMCs) derived from skeletal myoblasts. Initially, myoblasts expressed all myogenic cell-related markers such as Myf5, MyoD and Myogenin and differentiate into myotubes. After culture in a medium containing vascular endothelial growth factor (VEGF), these cells were shown to have adopted a differentiated SMC identity as demonstrated by αSMA, SM22α, calponin and smooth muscle-myosin heavy chain expression. Moreover, the cells cultured in the presence of VEGF did not express MyoD anymore and were unable to fuse in multinucleated myotubes. We demonstrated that myoblasts-derived SMCs (MDSMCs) interacted with endothelial cells to form, in vitro , a capillary-like network in three-dimensional collagen culture and, in vivo , a functional vascular structure in a Matrigel implant in nonobese diabetic-severe combined immunodeficient mice. Based on the easily available tissue source and their differentiation into functional SMCs, these data argue that skeletal myoblasts might represent an important tool for SMCs-based cell therapy.
- Subjects :
- Vascular Endothelial Growth Factor A
medicine.medical_specialty
Myoblasts, Skeletal
Calponin
Muscle Fibers, Skeletal
Myocytes, Smooth Muscle
Transplantation, Heterologous
Neovascularization, Physiologic
Mice, SCID
MyoD
Tissue Culture Techniques
Mice
Mice, Inbred NOD
Internal medicine
medicine
Myocyte
Animals
Humans
Myogenin
Cells, Cultured
Matrigel
biology
Myogenesis
Cell Differentiation
Epithelial Cells
Cell Biology
musculoskeletal system
Cell biology
Drug Combinations
Endocrinology
biology.protein
MYF5
Female
Proteoglycans
Collagen
Laminin
tissues
C2C12
Biomarkers
Subjects
Details
- ISSN :
- 00144827
- Volume :
- 313
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Experimental cell research
- Accession number :
- edsair.doi.dedup.....2667964fdc41cdc08ee7a8df8abe4fbb