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Transgenic overexpression of human LY6K in mice suppresses mature T cell development in the thymus

Transgenic overexpression of human LY6K in mice suppresses mature T cell development in the thymus

Authors :
Yesol Kim
Hyun Kyung Kong
Jong Hoon Park
Han Woong Lee
Dasom Son
Hyong Pyo Kim
Min Ji Song
Source :
Oncology Letters.
Publication Year :
2018
Publisher :
Spandidos Publications, 2018.

Abstract

Lymphocyte antigen 6 family member K (LY6K) is upregulated in a number of types of cancer and promotes tumor cell proliferation and metastasis. In addition, LY6K is involved in tamoxifen resistance in breast cancer. However, the in vivo molecular mechanism of LY6K has not yet been investigated. In the present study, transgenic mice overexpressing human LY6K (hLY6K) were generated using the pMAMneo vector, and the effect of LY6K upregulation in vivo was investigated. A total of 4 transgenic mice were generated, and the gene copy number was examined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). RT-qPCR demonstrated that mRNA of hLY6K was overexpressed in the thymus and spleen of the transgenic mice compared with wild-type mice. Flow cytometric analysis demonstrated that the proportions of B and T cells in the spleen were similar in wild-type and transgenic mice; however, the proportion of thymic mature T cells decreased in the transgenic mice, while there was an increase in the proportion of naïve T cells. These findings suggest that the overexpression of LY6K suppresses T cell development, and that LY6K is a potential therapeutic target for cancer.

Details

ISSN :
17921082 and 17921074
Database :
OpenAIRE
Journal :
Oncology Letters
Accession number :
edsair.doi.dedup.....2673ac654dfeb389842c1b39e8785dd9
Full Text :
https://doi.org/10.3892/ol.2018.9548