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Multiplane differential phase contrast imaging using asymmetric illumination in volume holographic microscopy
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2020
- Publisher :
- Society of Photo-Optical Instrumentation Engineers, 2020.
-
Abstract
- Significance: Differential phase contrast (DPC) is a well-known imaging technique for phase imaging. However, simultaneously acquiring multidepth DPC images is a non-trivial task. We propose simultaneous multiplane DPC imaging using volume holographic microscopy (VHM). Aim: To design and implement a new configuration of DPC-VHM for multiplane imaging. Approach: The angularly multiplexed volume holographic gratings (AMVHGs) and the wavelength-coded volume holographic gratings (WC-VHGs) are used for this purpose. To obtain asymmetric illumination for DPC images, a dynamic illumination system is designed by modifying the regular Köhler illumination using a thin film transistor panel (TFT-panel). Results: Multidepth DPC images of standard resolution chart and biosamples were used to compare imaging performance with the corresponding bright-field images. An average contrast enhancement of around three times is observed for target resolution chart by DPC-VHM. Imaging performance of our system is studied by modulation transfer function analysis, which suggests that DPC-VHM not only suppresses the DC component but also enhances high-frequency information. Conclusions: Proposed DPC-VHM can acquire multidepth-resolved DPC images without axial scanning. The illumination part of the system is adjustable so that the system can be adapted to bright-field mode, phase contrast mode, and DPC mode by controlling the pattern on the TFT-panel.
- Subjects :
- Paper
Microscope
Materials science
illumination design
Biomedical Engineering
Holography
imaging systems
01 natural sciences
law.invention
010309 optics
Biomaterials
Optics
law
Optical transfer function
0103 physical sciences
Microscopy
Köhler illumination
Microscopy, Phase-Contrast
Special Series on Biomedical Imaging and Sensing
volume gratings
Diffraction grating
Lighting
business.industry
Diagnostic Tests, Routine
Resolution (electron density)
diffraction gratings
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
business
DC bias
Subjects
Details
- Language :
- English
- ISSN :
- 15602281 and 10833668
- Volume :
- 25
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....26900bb3c98356a693332f96cb592b68