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Comparative Analysis of the Circular and Highly Asymmetrical Marseilleviridae Genomes
- Source :
- Viruses, Volume 12, Issue 11, Viruses, MDPI, 2020, 12 (11), pp.1270. ⟨10.3390/v12111270⟩, Viruses, Vol 12, Iss 1270, p 1270 (2020), Viruses, 2020, 12 (11), pp.1270. ⟨10.3390/v12111270⟩
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Marseilleviridae members are large dsDNA viruses with icosahedral particles 250 nm in diameter infecting Acanthamoeba. Their 340 to 390 kb genomes encode 450 to 550 protein-coding genes. Since the discovery of marseillevirus (the prototype of the family) in 2009, several strains were isolated from various locations, among which 13 are now fully sequenced. This allows the organization of their genomes to be deciphered through comparative genomics. Here, we first experimentally demonstrate that the Marseilleviridae genomes are circular. We then acknowledge a strong bias in sequence conservation, revealing two distinct genomic regions. One gathers most Marseilleviridae paralogs and has undergone genomic rearrangements, while the other, enriched in core genes, exhibits the opposite pattern. Most of the genes whose protein products compose the viral particles are located in the conserved region. They are also strongly biased toward a late gene expression pattern. We finally discuss the potential advantages of Marseilleviridae having a circular genome, and the possible link between the biased distribution of their genes and the transcription as well as DNA replication mechanisms that remain to be characterized.
- Subjects :
- 0301 basic medicine
Comparative genomics
Genome evolution
biology
Marseilleviridae
030106 microbiology
lcsh:QR1-502
Marseillevirus
DNA replication
marseillevirus
comparative genomics
genome evolution
biology.organism_classification
Genome
lcsh:Microbiology
03 medical and health sciences
large DNA viruses
030104 developmental biology
Infectious Diseases
Transcription (biology)
Evolutionary biology
Virology
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Gene
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....26a2e1b604c819ff27b100ab09178784
- Full Text :
- https://doi.org/10.3390/v12111270