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Accurate determination of the CYP2D6 (*1/*4)xN genotype by quantitative PCR
- Source :
- Drug Metabolism and Personalized Therapy, 33(1), 33-39. De Gruyter
- Publication Year :
- 2018
- Publisher :
- De Gruyter, 2018.
-
Abstract
- Background:CYP2D6is responsible for the metabolism of approximately 25% of all drugs. The expression of cytochrome P450 2D6 (CYP2D6) is influenced by a combination of factors including polymorphisms in theCYP2D6gene. Analysis of theCYP2D6genotype is used to personalize the medication to a patient’s metabolism. Although many genotypes can be determined using standard genotype analysis, in some cases, an incomplete analysis is performed. TheCYP2D6genotype *1/*4 often occurs in combination with a multiplication of theCYP2D6gene, and is reported as (*1/*4)xN. Accurate determination of the multiplied gene is essential to provide a phenotype prediction for these patients. Duplication of the *1 gene leads to an extensive metabolizer genotype whereas multiplication of the *4 gene would not lead to extra functional enzyme and therefore provides an intermediate metabolizer phenotype.Methods:Here, a technique is described in which the copy numbers of both the *4 and *1 genes are determined using quantitative PCR techniques.Results and conclusions:This technique provides a method to predict the patient’sCYP2D6phenotype, and is therefore an important step toward personalized medicine.
- Subjects :
- Genetics
CYP2D6
Genotyping Techniques
Gene Dosage
CYP2D6 Gene
Biology
Real-Time Polymerase Chain Reaction
Gene dosage
Phenotype
Sensitivity and Specificity
Cytochrome P-450 CYP2D6
Gene duplication
Genotype
Humans
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
Gene
Pharmacogenetics
Alleles
Subjects
Details
- ISSN :
- 23638915 and 23638907
- Volume :
- 33
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Drug Metabolism and Personalized Therapy
- Accession number :
- edsair.doi.dedup.....26aa4128f87a991503ef1b7ceff865c4