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Rapid screening of marine bacterial symbionts using MALDI-TOF MS
- Source :
- Archives of microbiology. 202(8)
- Publication Year :
- 2020
-
Abstract
- Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including Vibrio, Bacillus, Pseudovibrio, Alteromonas and Ruegeria. MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% pyrH gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for Vibrio species groups’ identification.
- Subjects :
- Ruegeria
Marine Biology
Mass spectrometry
Biochemistry
Microbiology
03 medical and health sciences
RNA, Ribosomal, 16S
Genetics
Environmental Microbiology
Seawater
Alteromonas
Symbiosis
Molecular Biology
030304 developmental biology
Vibrio
0303 health sciences
biology
030306 microbiology
Chemistry
General Medicine
biology.organism_classification
16S ribosomal RNA
Matrix-assisted laser desorption/ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Pseudovibrio
Bacteria
Subjects
Details
- ISSN :
- 1432072X
- Volume :
- 202
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Archives of microbiology
- Accession number :
- edsair.doi.dedup.....26b11a7b6039c65ea763dd1f48650fe8