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Rapid induction of gliogenesis in OLIG2 and NKX2.2‐expressing progenitors‐derived spheroids
- Source :
- Stem Cells Translational Medicine
- Publication Year :
- 2020
- Publisher :
- John Wiley & Sons, Inc., 2020.
-
Abstract
- Glial cells are crucial for the development of the central nervous system and the maintenance of chemical homeostasis. The process of gliogenesis has been well studied in the rodent brain, but it remains less well studied in the human brain. In addition, rodent glial cells differ from human counterparts in terms of morphologies, functions, and anatomical locations. Cerebral organoids (also referred to as spheroids) derived from human pluripotent stem cells (hPSCs) have been developed and are suitable cell‐based models for researching developmental and neurodegenerative diseases. The in vitro generation of glia, including astrocytes and oligodendrocytes, from such organoids represents a promising tool to model neuronal diseases. Here, we showed that three‐dimensional (3D) culture of OLIG2‐ and NKX2.2‐expressing neurospheres produced efficiently mature astrocytes and oligodendrocytes in terms of morphologies and expression pattern recapitulating native 3D environment. Our findings provide important insights for developmental research of the human brain and glial specification that may facilitate patient‐specific disease modeling.<br />Human pluripotent stem cell‐derived cerebral organoids can mimic development of the mammalian central nervous system. In this study, we demonstrate that pre‐patterned stem/progenitor cells, especially OLIG2/NKX2.2‐expressing pre‐oligodendrocyte progenitor cells, facilitate glial specification during organoid development. The resulting spheroids can serve as in vitro model for myelination to evaluate promyelination drugs and a source for cell therapy in demyelinating disease.
- Subjects :
- 0301 basic medicine
Pluripotent Stem Cells
glia
induced pluripotent stem cells
oligodendrocytes
Biology
drug target
OLIG2
03 medical and health sciences
0302 clinical medicine
Tissue‐specific Progenitor and Stem Cells
Neurosphere
Spheroids, Cellular
medicine
Humans
Progenitor cell
Induced pluripotent stem cell
Gliogenesis
Homeodomain Proteins
Nuclear Proteins
Cell Biology
General Medicine
Human brain
embryonic stem cells
Oligodendrocyte Transcription Factor 2
Zebrafish Proteins
Embryonic stem cell
Cell biology
030104 developmental biology
medicine.anatomical_structure
Homeobox Protein Nkx-2.2
Chemical homeostasis
030217 neurology & neurosurgery
Developmental Biology
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 21576580 and 21576564
- Volume :
- 9
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Stem Cells Translational Medicine
- Accession number :
- edsair.doi.dedup.....26c70205cd801c6cb5fbdf5a04d9cebf