Back to Search
Start Over
Grape seed proanthocyanidins protect against streptozotocin‑induced diabetic nephropathy by attenuating endoplasmic reticulum stress‑induced apoptosis
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2018
- Publisher :
- Spandidos Publications, 2018.
-
Abstract
- Diabetic nephropathy (DN) is by far the most common cause of end-stage renal disease (ESRD) in industrial countries, accounting for ~45% of all new ESRD cases in the United States. Grape seed proanthocyanidin extracts (GSPE) are powerful antioxidants, with an antioxidant ability 50-fold greater than that of vitamin E and 20-fold greater than that of vitamin C. The present study investigated whether GSPE can protect against streptozotocin (STZ)-induced DN and aimed to elucidate a possible mechanism. Male Sprague Dawley rats were randomly divided into three groups: Control group (N), diabetes mellitus group (DM) injected with 40 mg/kg STZ, and the GSPE treatment group (intragastric administration of 250 mg/kg/day GSPE for 16 weeks after diabetes was induced in the rats). Blood and kidney samples were collected after treatment. The renal pathological changes were determined with periodic acid-Schiff (PAS) staining, while the protein expression levels of glucose-regulated protein 78 (GRP78), phosphorylated-extracellular signal-regulated kinase (p-ERK) and Caspase-12 were determined by western blotting and immunohistochemical staining. Apoptosis was determined with a terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Compared with the DM group, the GSPE group had no significant changes in the blood urea nitrogen (BUN) level and serum creatinine (Scr) level, but showed a significant decline in the renal index (RI) level and 24-h urinary albumin level (P
- Subjects :
- Blood Glucose
Male
0301 basic medicine
Cancer Research
medicine.medical_treatment
Apoptosis
Kidney
Biochemistry
Antioxidants
Blood Urea Nitrogen
Rats, Sprague-Dawley
Diabetic nephropathy
chemistry.chemical_compound
0302 clinical medicine
Diabetic Nephropathies
Phosphorylation
Endoplasmic Reticulum Chaperone BiP
Blood urea nitrogen
Caspase 12
Heat-Shock Proteins
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
TUNEL assay
Articles
Endoplasmic Reticulum Stress
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Molecular Medicine
medicine.drug
medicine.medical_specialty
streptozotocin
Drug Administration Schedule
Streptozocin
Diabetes Mellitus, Experimental
03 medical and health sciences
Albumins
Internal medicine
Genetics
medicine
Animals
Humans
Proanthocyanidins
Molecular Biology
Gastric Absorption
Creatinine
Grape Seed Extract
business.industry
diabetic nephropathy
Vitamin E
Kidney metabolism
Epithelial Cells
Streptozotocin
medicine.disease
Rats
030104 developmental biology
Endocrinology
Gene Expression Regulation
chemistry
grape seed proanthocyanidins
business
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....26e0fc7fcc6ce26e965fec249a9efc2f
- Full Text :
- https://doi.org/10.3892/mmr.2018.9140