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Consistent high concentration of the viral microRNA BART17 in plasma samples from nasopharyngeal carcinoma patients - evidence of non-exosomal transport
- Source :
- Virology Journal, Virology Journal, BioMed Central, 2013, 10 (1), pp.119. 〈10.1186/1743-422X-10-119〉, Virology Journal, 2013, 10 (1), pp.119. ⟨10.1186/1743-422X-10-119⟩, Virology Journal, BioMed Central, 2013, 10 (1), pp.119. ⟨10.1186/1743-422X-10-119⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Background Because latent Epstein Barr (EBV)-infection is a specific characteristic of malignant nasopharyngeal carcinoma (NPC), various molecules of viral origin are obvious candidate biomarkers in this disease. In a previous study, we could show in a few clinical samples that it was possible to detect a category of EBV microRNAs called miR-BARTs in the plasma of at least a fraction of NPC patients. The first aim of the present study was to investigate the status of circulating miR-BART17-5p (one of the miR-BARTs hereafter called miR-BART17) and EBV DNA in a larger series of NPC plasma samples. The second aim was to determine whether or not circulating miR-BART17 was carried by plasma exosomes. Patients and methods Plasma samples were collected from 26 NPC patients and 10 control donors, including 9 patients with non-NPC Head and Neck squamous cell carcinoma and one healthy EBV carrier. Concentrations of miR-BART17 and two cellular microRNAs (hsa-miR-16 and -146a) were assessed by real-time quantitative PCR with spike-in normalization and absolute quantification. In addition, for 2 patients, exosome distributions of miR-BART17 and miR-16 were investigated following plasma lipoprotein fractionation by isopycnic density gradient ultrcentrifugation. Results The miR-BART17 was significantly more abundant in plasma samples from NPC patients compared to non-NPC donors. Above a threshold of 506 copies/mL, detection of miR-BART17 was highly specific for NPC patients (ROC curve analysis: AUC=0.87 with true positive rate = 0.77, false positive rate = 0.10). In this relatively small series, the concentration of plasma miR-BART17 and the plasma EBV DNA load were not correlated. When plasma samples were fractionated, miR-BART17 co-purified with a protein-rich fraction but not with exosomes. Conclusions Detection of high concentrations of plasma miR-BART17 is consistent in NPC patients. This parameter is, at least in part, independent of the viral DNA load. Circulating miR-BART17 does not co-purify with exosomes.
- Subjects :
- Male
Herpesvirus 4, Human
medicine.disease_cause
Exosomes
Plasma
0302 clinical medicine
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
0303 health sciences
microRNA
Middle Aged
3. Good health
[ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Real-time polymerase chain reaction
Infectious Diseases
030220 oncology & carcinogenesis
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
RNA, Viral
Female
Adult
Lipoproteins
Nasopharyngeal neoplasm
miR-BART
Biology
Real-Time Polymerase Chain Reaction
Exosome
03 medical and health sciences
Virology
Head and Neck carcinomas
medicine
Carcinoma
Nasopharyngeal carcinoma
Humans
Epstein-Barr virus
030304 developmental biology
Aged
Research
Biological Transport
Nasopharyngeal Neoplasms
Biomarker
medicine.disease
Epstein–Barr virus
Head and neck squamous-cell carcinoma
Microvesicles
MicroRNAs
DNA, Viral
Biomarkers
DNA load
Subjects
Details
- Language :
- English
- ISSN :
- 1743422X
- Database :
- OpenAIRE
- Journal :
- Virology Journal, Virology Journal, BioMed Central, 2013, 10 (1), pp.119. 〈10.1186/1743-422X-10-119〉, Virology Journal, 2013, 10 (1), pp.119. ⟨10.1186/1743-422X-10-119⟩, Virology Journal, BioMed Central, 2013, 10 (1), pp.119. ⟨10.1186/1743-422X-10-119⟩
- Accession number :
- edsair.doi.dedup.....26f8adf2fae9c7ebbb9f3d1de64f7133