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Implication of fermentable carbohydrates targeting the gut microbiota on conjugated linoleic acid production in high-fat-fed mice
Implication of fermentable carbohydrates targeting the gut microbiota on conjugated linoleic acid production in high-fat-fed mice
- Source :
- The British Journal of Nutrition : an international journal of nutritional science, Vol. 110, no. 6, p. 998-1011 (2013), Vrije Universiteit Brussel
- Publication Year :
- 2013
- Publisher :
- Cambridge University Press (CUP), 2013.
-
Abstract
- In vitro experiments have shown that isolated human gut bacteria are able to metabolise PUFA into conjugated PUFA like conjugated linoleic acids (CLA). The hypothesis of the present paper was that high-fat (HF) diet feeding and supplementation with fermentable carbohydrates that have prebiotic properties modulate the in vivo production of CLA by the mouse gut microbiota. Mice were treated for 4 weeks as follows: control (CT) groups were fed a standard diet; HF groups were fed a HF diet rich in linoleic acid (18 : 2n-6); the third groups were fed with the HF diet supplemented with either inulin-type fructans (HF-ITF) or arabinoxylans (HF-Ax). HF diet feeding increased rumenic acid (cis-9, trans-11-18 : 2 CLA) content both in the caecal and liver tissues compared with the CT groups. ITF supplementation had no major effect compared with the HF diet whereas Ax supplementation increased further rumenic acid (cis-9, trans-11-18 : 2 CLA) in the caecal tissue. These differences between both prebiotics may be linked to the high fat-binding capacity of Ax that provides more substrates for bacterial metabolism and to differential modulation of the gut microbiota (specific increase in Roseburia spp. in HF-Ax v. HF). In conclusion, these experiments supply the proof of concept that the mouse gut microbiota produces CLA in vivo, with consequences on the level of CLA in the caecal and liver tissues. We postulate that the CLA-producing bacteria could be a mediator to consider in the metabolic effects of both HF diet feeding and prebiotic supplementation.
- Subjects :
- Male
medicine.medical_treatment
Conjugated linoleic acid
Linoleic acid
Carbohydrates
Medicine (miscellaneous)
Vaccenic acid
Gut microbiota
Biology
Gut flora
Carbohydrate metabolism
digestive system
Gene Expression Regulation, Enzymologic
Mice
chemistry.chemical_compound
Fatty Acids, Omega-6
medicine
Animals
Linoleic Acids, Conjugated
Food science
Prebiotic supplementation
chemistry.chemical_classification
Nutrition and Dietetics
Bacteria
Rumenic acid
Prebiotic
food and beverages
biology.organism_classification
Dietary Fats
Intestines
Mice, Inbred C57BL
high-fat diet
Prebiotics
Liver
chemistry
Fermentation
Carbohydrate Metabolism
Conjugated linoleic acids
Stearoyl-CoA Desaturase
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 14752662 and 00071145
- Volume :
- 110
- Database :
- OpenAIRE
- Journal :
- British Journal of Nutrition
- Accession number :
- edsair.doi.dedup.....270f01a0116f2d75dd45716b7652d36c
- Full Text :
- https://doi.org/10.1017/s0007114513000123