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Differential regulation of thyrotropin-releasing hormone receptor mRNA levels by thyroid hormone in vivo and in vitro (GH3 cells)
- Source :
- Biochemical and biophysical research communications. 184(1)
- Publication Year :
- 1992
-
Abstract
- We studied the effect of thyroid status on thyrotropin-releasing hormone receptor (TRH-R) mRNA levels both in vivo and in vitro (GH3 cells) using a cloned rat TRH-R cDNA by RT-PCR. Experimental hypothyroid rats were produced by total thyroidectomy and were then killed 7 days after the operation. TRH receptor binding in the anterior pituitary and serum TSH level were elevated approximately 2-fold and 8-fold, respectively, in 7 day thyroidectomized rats. TRH-R mRNA levels in hypothyroid rats were also increased significantly compared with those of normal rats. In GH3 cells, however, no significant change of TRH-R mRNA level was observed between cultures treated with triiodothyronine (T3, 10 −9 and 10 −7 M) and the untreated group. The present data indicate that 1) the in vivo effects of thyroid status on TRH-R mRNA levels differ from the in vitro one, and that 2) the down regulation ofTTRH-R binding by thyroid hormone in GH3 cells may be mediated by translational or post-translational mechanisms.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
endocrine system diseases
Molecular Sequence Data
Biophysics
Down-Regulation
Gene Expression
Peptide hormone
Biology
Biochemistry
Polymerase Chain Reaction
Cell Line
Anterior pituitary
Hypothyroidism
In vivo
Thyrotropin-releasing hormone receptor
Pituitary Gland, Anterior
Internal medicine
medicine
Animals
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Molecular Biology
Thyrotropin-Releasing Hormone
Triiodothyronine
Base Sequence
Receptors, Thyrotropin-Releasing Hormone
Thyroid
Rats, Inbred Strains
Cell Biology
Blotting, Northern
Rats
Receptors, Neurotransmitter
Endocrinology
medicine.anatomical_structure
Oligodeoxyribonucleotides
Hormone receptor
Thyroidectomy
RNA
Poly A
hormones, hormone substitutes, and hormone antagonists
Hormone
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 184
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....277daceedf58a2cca038a4760e04df1f