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The Labeling, Visualization, and Quantification of Hyaluronan Distribution in Tumor-Bearing Mouse Using PET and MR Imaging
- Source :
- Pharmaceutical Research. 37
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Hyaluronan (HA) based biomaterials are widely used as tissue scaffolds, drug formulations, as well as targeting ligands and imaging probes for diagnosis and drug delivery. However, because of the presence of abundant endogenous HA presented in various tissues in vivo, the pharmacokinetic behavior and biodistribution patterns of exogenously administered HAs have not been well characterized. The HA backbone was modified with Diethylenetriamine (DTPA) to enable the chelation of gadolinium (Gd) and aluminum (Al) ions. Series of PET and MR imaging were taken after the injection of HA-DTPA-Gd and HA-DTPA-Al18F while using18F-FDG and Magnevist(DTPA-Gd) as controls. The Tomographic images were analyzed and quantified to reveal the distribution and locations of HA in tumor-bearing mice. The labeled HAs had good stability in plasma. They retained binding affinity towards CD44s on tumor cell surface. The injected HAs distributed widely in various organs, but were found to be cleared quickly except inside tumor tissues where the signals were higher and persisted longer. Medical imaging tools, including MR and PET, can be highly valuable for examining biomaterial distribution non-invasively. The HA tumor accumulation properties may be explored for the development of active targeting drug carriers and molecular probes.
- Subjects :
- Male
Biodistribution
Gadolinium
Contrast Media
Pharmaceutical Science
chemistry.chemical_element
Biocompatible Materials
02 engineering and technology
030226 pharmacology & pharmacy
Mice
03 medical and health sciences
0302 clinical medicine
Pharmacokinetics
In vivo
Neoplasms
Animals
Humans
Distribution (pharmacology)
Tissue Distribution
Pharmacology (medical)
Hyaluronic Acid
Pharmacology
Drug Carriers
Organic Chemistry
Biomaterial
021001 nanoscience & nanotechnology
Magnetic Resonance Imaging
Xenograft Model Antitumor Assays
chemistry
A549 Cells
Molecular Probes
Positron-Emission Tomography
Drug delivery
Molecular Medicine
0210 nano-technology
Drug carrier
Biotechnology
Biomedical engineering
Subjects
Details
- ISSN :
- 1573904X and 07248741
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical Research
- Accession number :
- edsair.doi.dedup.....278b1c89550da7c50ffce7dc8a4b74ab