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ABCA1 Is Coordinated with ABCB1 in the Arsenic-Resistance of Human Cells
- Source :
- Applied biochemistry and biotechnology. 187(1)
- Publication Year :
- 2018
-
Abstract
- Arsenic is one of the most widespread global environmental toxicants associated with endemic poisoning. ATP-binding cassette (ABC) proteins are transmembrane channels that transport and dispose of lipids and metabolic products across the plasma membrane. The majority of ABC family members (including ABCB1 and ABCC1) are reported to play a role in the development of arsenic and drug resistance in mammals. Previously, we established a human arsenic-resistant ECV-304 (AsRE) cell line and identified ABCA1 as a novel arsenic resistance gene. In the current study, we further investigated the potential contribution of ABCA1, ABCB1, and ABCC1 to arsenic resistance through measurement of survival rates and arsenic accumulation in AsRE cells with RNA interference. The arsenic resistance capacity of ABCC1 was the strongest among the three genes, while those of ABCA1 and ABCB1 were similar. Double or triple gene knockdown of ABCA1, ABCB1, and ABCC1 via RNA interference led to a decrease significant in arsenic resistance when ABCA1/ABCB1 or ABCB1/ABCC1 were simultaneously silenced. Interestingly, no differences were evident between cells with ABCA1/ABCC1 and ABCC1 only knockdown. Our findings suggest that ABCA1 and ABCB1 proteins display similar arsenic resistance capabilities and possibly coordinate to promote arsenic resistance in AsRE cells.
- Subjects :
- inorganic chemicals
0301 basic medicine
ATP Binding Cassette Transporter, Subfamily B
Drug Resistance
chemistry.chemical_element
Bioengineering
Drug resistance
Applied Microbiology and Biotechnology
Biochemistry
Arsenic
Cell Line
03 medical and health sciences
0302 clinical medicine
RNA interference
Humans
Molecular Biology
Gene
Gene knockdown
integumentary system
biology
Chemistry
Biological Transport
General Medicine
Cell biology
030104 developmental biology
Cell culture
030220 oncology & carcinogenesis
ABCA1
Gene Knockdown Techniques
biology.protein
ABCC1
lipids (amino acids, peptides, and proteins)
RNA Interference
Multidrug Resistance-Associated Proteins
Biotechnology
ATP Binding Cassette Transporter 1
Subjects
Details
- ISSN :
- 15590291
- Volume :
- 187
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Applied biochemistry and biotechnology
- Accession number :
- edsair.doi.dedup.....27c4033afdfbff5b1c2527d3656f2882