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Persistence of Leptospira borgpetersenii Serovar Hardjo in Refrigerated Raw Milk: A Transmission Risk of Leptospirosis to Humans
- Source :
- Pathogens, Pathogens; Volume 10; Issue 3; Pages: 291, Pathogens, Vol 10, Iss 291, p 291 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Leptospira borgpetersenii serovar Hardjo (LH) is an important infectious agent of reproduction pathologies and lactation decline in cattle, with a possible zoonotic role. To figure out the potential zoonotic risk for human raw-milk consumption, the present study aims at assessing the persistence and viability of LH in refrigerated raw milk over a 10-day period, which is set as the maximum time range for raw-milk domestic consumption. A negative sample of fresh raw milk was contaminated with an LH strain (2 × 108 Leptospires/mL) and analyzed by a rrs (16S) gene targeting real-time PCR (rPCR) protocol for LH DNA at days 1, 2, 3, 6, 7, 9, and 10. Seven aliquots of the same sampling time were inoculated into a semisolid EMJH media for bacterial culture. All aliquots tested positive in both rPCR and culture, which demonstrates that raw milk does not alter the detectability and viability of LH, respectively. The analytical sensitivity (LoD, limit of detection) determined for the rPCR (103 Leptospires/mL) was repeatable during the study, whereas it gradually decreased when it came to the bacterial culture. This study demonstrates that bovine raw milk might be a potential vehicle of infection by LH, even when storage conditions are strictly respected.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Serotype
Veterinary medicine
Microbiological culture
media_common.quotation_subject
bovine milk
030106 microbiology
raw consumption
lcsh:Medicine
Biology
Article
Persistence (computer science)
03 medical and health sciences
0302 clinical medicine
fluids and secretions
Lactation
medicine
Immunology and Allergy
leptospirosis
Leptospira Hardjo (LH) persistence
Molecular Biology
media_common
General Immunology and Microbiology
Inoculation
lcsh:R
Raw milk
medicine.disease
Leptospirosis
Infectious Diseases
medicine.anatomical_structure
Reproduction
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 20760817
- Volume :
- 10
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Pathogens
- Accession number :
- edsair.doi.dedup.....27d873f890cbe65dc5774283e1c9f351