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An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells

Authors :
Arno van Cruchten
L IJlst
Hans R. Waterham
Carlo W.T. van Roermund
Katharina Herzog
Ronald J.A. Wanders
Wim Kulik
Laboratory Genetic Metabolic Diseases
AGEM - Inborn errors of metabolism
APH - Methodology
ARD - Amsterdam Reproduction and Development
Source :
Metabolites, Vol 9, Iss 3, p 45 (2019), Metabolites, Metabolites, 9(3):45. Multidisciplinary Digital Publishing Institute
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).

Details

Language :
English
ISSN :
22181989
Volume :
9
Issue :
3
Database :
OpenAIRE
Journal :
Metabolites
Accession number :
edsair.doi.dedup.....27e8865542eb75601c40bf93430c1fb9