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An UPLC-MS/MS Assay to Measure Glutathione as Marker for Oxidative Stress in Cultured Cells
- Source :
- Metabolites, Vol 9, Iss 3, p 45 (2019), Metabolites, Metabolites, 9(3):45. Multidisciplinary Digital Publishing Institute
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).
- Subjects :
- Antioxidant
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Cell
lcsh:QR1-502
yeast
medicine.disease_cause
01 natural sciences
Biochemistry
High-performance liquid chromatography
lcsh:Microbiology
03 medical and health sciences
chemistry.chemical_compound
medicine
Protocol
oxidative stress
glutathione
Molecular Biology
030304 developmental biology
mass spectrometry
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
Chemistry
010401 analytical chemistry
cultured cells
Glutathione
Yeast
0104 chemical sciences
3. Good health
medicine.anatomical_structure
Ageing
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 22181989
- Volume :
- 9
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Metabolites
- Accession number :
- edsair.doi.dedup.....27e8865542eb75601c40bf93430c1fb9