DNA barcoding, MALDI-TOF, and AFLP data support Fusarium ficicrescens as a distinct species within the Fusarium fujikuroi species complex

The Fusarium fujikuroi species complex (FFSC) is one of the most common group of fusaria associated with plant diseases, mycotoxins production and traumatic and disseminated human infections. Here we present the description and taxonomy of a new taxon, Fusarium ficicrescens sp. nov., collected from contaminated fig fruits in Iran. Initially this species identified as F. andiyazi by morphology. In the present study the species was studied by multilocus sequence analysis, amplified fragment length polymorphism (AFLP), matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and phenotypic characters. Multilocus analyses were based on translation elongation factor 1α (TEF1), RNA polymerase subunit (RPB2) and beta-tubulin (BT2) and proved F. ficicrescens as a member of the FFSC. Phylogenetic analysis showed that the fungus is closely related to F. lactis, F. ramigenum, and F. napiforme; known plant pathogens, mycotoxin producers, and occasionally occurring multidrug resistant opportunists. The new species differed by being able to grow at 37ºC and by the absence of mycotoxin production. TEF1 was confirmed as an essential barcode for identifying Fusarium species. In addition to TEF1, we evaluated BT2 and RPB2 in order to provide sufficient genetic and species boundaries information for recognition of the novel species. DNA barcoding, MALDI-TOF and AFLP data support Fusarium ficicrescens as a distinct species within the F. fujikuroi species complex (PDF Download Available). Available from: https://www.researchgate.net/publication/280444385_DNA_barcoding_MALDI-TOF_and_AFLP_data_support_Fusarium_ficicrescens_as_a_distinct_species_within_the_F_fujikuroi_species_complex [accessed Jan 11, 2016].