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OsTIR1 and OsAFB2 Downregulation via OsmiR393 Overexpression Leads to More Tillers, Early Flowering and Less Tolerance to Salt and Drought in Rice
- Source :
- PLoS ONE, Vol 7, Iss 1, p e30039 (2012), PLoS ONE
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- The microRNA miR393 has been shown to play a role in plant development and in the stress response by targeting mRNAs that code for the auxin receptors in Arabidopsis. In this study, we verified that two rice auxin receptor gene homologs (OsTIR1 and OsAFB2) could be targeted by OsmiR393 (Os for Oryza sativa). Two new phenotypes (increased tillers and early flowering) and two previously observed phenotypes (reduced tolerance to salt and drought and hyposensitivity to auxin) were observed in the OsmiR393-overexpressing rice plants. The OsmiR393-overexpressing rice demonstrated hyposensitivity to synthetic auxin-analog treatments. These data indicated that the phenotypes of OsmiR393-overexpressing rice may be caused through hyposensitivity to the auxin signal by reduced expression of two auxin receptor genes (OsTIR1 and OsAFB2). The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering. The positive phenotypes (increased tillers and early flowering) and negative phenotypes (reduced tolerance to salt and hyposensitivity to auxin) of OsmiR393-overexpressing rice present a dilemma for molecular breeding.
- Subjects :
- Time Factors
2,4-Dichlorophenoxyacetic acid
lcsh:Medicine
Plant Science
Genetically modified crops
Sodium Chloride
Biochemistry
Naphthaleneacetic Acids
chemistry.chemical_compound
Gene Expression Regulation, Plant
Arabidopsis
Arabidopsis thaliana
heterocyclic compounds
lcsh:Science
Plant Proteins
Regulation of gene expression
chemistry.chemical_classification
Multidisciplinary
biology
food and beverages
Agriculture
Plants
Plants, Genetically Modified
Adaptation, Physiological
Droughts
Phenotype
Organ Specificity
2,4-Dichlorophenoxyacetic Acid
Signal Transduction
Research Article
Biotechnology
Down-Regulation
Cereals
Receptors, Cell Surface
Crops
Flowers
Genes, Plant
Real-Time Polymerase Chain Reaction
Models, Biological
Model Organisms
Plant and Algal Models
Auxin
Sequence Homology, Nucleic Acid
Botany
RNA, Messenger
Biology
Gene
Oryza sativa
Indoleacetic Acids
Gene Expression Profiling
lcsh:R
fungi
Reproducibility of Results
Oryza
biology.organism_classification
MicroRNAs
chemistry
Plant Biotechnology
lcsh:Q
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....28084a20218013725897a6afbea6e7a1