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Lysosome membrane permeability to anions
- Source :
- Biochimica et Biophysica Acta (BBA) - Biomembranes. 1373:17-26
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- The permeability of rat liver lysosomes to some inorganic and aliphatic organic anions was investigated, using an osmotic-protection methodology. Lysosomes were incubated at 25 degreesC in 250 mOsm solutions of potassium salts of the anions, in the presence of valinomycin, and the latency of lysosomal hexosaminidase measured at intervals. Lysosomes suspended in 250 mM sucrose at 25 degreesC were stable for up to 4 h. When suspended in 250 mOsm solutions of potassium salts of inorganic acids, latency was lost at rates indicating anion permeance decreasing in the order thiocyanate, nitrate and iodidebromidechloridesulfate. This rank order does not correspond with the anion selectivity of any known anion transporter, and is closer to that of the lyotropic series. Results with the potassium salts of aliphatic organic acids indicate little correlation between permeation and hydrocarbon chain length, although formate was more rapidly permeant than acetate and its higher homologs. By contrast, oxalate was less permeable than other dicarboxylic acids. The presence of one or more hydroxy groups decreased permeance. A correlation between permeance and the acid's lowest pKa suggested that penetration was due principally to the entry of the undissociated acid, but there is evidence that the (much more abundant) singly charged anionic form is also significantly permeant.
- Subjects :
- Anions
Cell Membrane Permeability
Membrane permeability
Inorganic chemistry
Biophysics
Anion, inorganic
Lysosome membrane
Ether
Biochemistry
chemistry.chemical_compound
Lysosome
Polymer chemistry
Pyridine
medicine
Animals
Ion transporter
Ion Transport
biology
Chemistry
Osmolar Concentration
Anion, organic
Intracellular Membranes
Cell Biology
beta-N-Acetylhexosaminidases
Rats
medicine.anatomical_structure
Membrane
Liver
biology.protein
Amine gas treating
Lysosomes
Organic anion
Subjects
Details
- ISSN :
- 00052736
- Volume :
- 1373
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Accession number :
- edsair.doi.dedup.....28268a3f2d04bfeb4ae7ffd0c1d2db00