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Airway remodeling in allergen-challenged Brown Norway rats: distribution of proteoglycans
- Source :
- American Journal of Physiology-Lung Cellular and Molecular Physiology. 290:L1052-L1058
- Publication Year :
- 2006
- Publisher :
- American Physiological Society, 2006.
-
Abstract
- Proteoglycans (PG) have important effects on the mechanical properties of tissues and the phenotype of various structural cells. Little is known about changes in PG deposition in the airways in animal models of asthma. We studied changes in PG in the airway wall of Brown Norway rats sensitized to ovalbumin (OA) and exposed to repeated OA challenge. Control (Sal) animals were sensitized and challenged with saline. After the 3rd challenge, animals were killed and lungs fixed in formalin. Tissue sections were incubated with antibodies to the small, leucine-rich PG, decorin, and biglycan and collagen type I. Airways were classified according to basement membrane perimeter length (≤0.99, 1–2.99, and ≥3 mm). Decorin, biglycan, and collagen type I were increased in the airways of OA vs. Sal rats. Remodeling was most prominent in central airways. The distribution of PG differed with respect to the subepithelial vs. airway smooth muscle (ASM) vs. adventitial layer. Whereas biglycan was readily detected within the ASM, decorin and collagen were detected outside the ASM and especially in the adventitial layer. Differences in the distribution of these molecules within the layers of the airway wall may reflect their specific functional roles.
- Subjects :
- Male
Pulmonary and Respiratory Medicine
Respiratory Mucosa
Ovalbumin
Physiology
Decorin
medicine.disease_cause
Bordetella pertussis
Eosinophil Major Basic Protein
Allergen
Airway resistance
Adjuvants, Immunologic
Immunologic
Reference Values
Rats, Inbred BN
Physiology (medical)
Biglycan
medicine
Animals
Adjuvants
biology
Airway Resistance
Collagen
Proteoglycans
Rats
Allergens
Cell Biology
respiratory system
Phenotype
Inbred BN
respiratory tract diseases
Cell biology
carbohydrates (lipids)
Immunology
Major basic protein
biology.protein
Subjects
Details
- ISSN :
- 15221504 and 10400605
- Volume :
- 290
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Accession number :
- edsair.doi.dedup.....2859a7a1b30aa98e782f48dc439a2d66