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Alpha‐ and beta‐mannan utilization by marine Bacteroidetes
- Source :
- Avci, B 2018, ' Alpha- and beta-mannan utilization by marine Bacteroidetes ', Environmental Microbiology, vol. 20, no. 11 . https://doi.org/10.1111/1462-2920.14414
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest α- and β-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for α- and β-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.
- Subjects :
- Proteomics
0301 basic medicine
Polysaccharide
Microbiology
Carbon Cycle
Mannans
03 medical and health sciences
Marine bacteriophage
Bacterial Proteins
Algae
Humans
Organic matter
Ecology, Evolution, Behavior and Systematics
Mannan
chemistry.chemical_classification
biology
Bacteroidetes
Carbon fixation
biology.organism_classification
030104 developmental biology
chemistry
Biochemistry
Carbohydrate Metabolism
North Sea
Bacteroides
Subjects
Details
- ISSN :
- 14622920 and 14622912
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....2873b058cf14bbc27aee26ae9d1cdce6