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Quantification of N-acetylcysteamine activated methylmalonate incorporation into polyketide biosynthesis
- Source :
- Beilstein Journal of Organic Chemistry, Beilstein Journal of Organic Chemistry, Vol 9, Iss 1, Pp 664-674 (2013)
- Publication Year :
- 2013
- Publisher :
- Beilstein-Institut, 2013.
-
Abstract
- Polyketides are biosynthesized through consecutive decarboxylative Claisen condensations between a carboxylic acid and differently substituted malonic acid thioesters, both tethered to the giant polyketide synthase enzymes. Individual malonic acid derivatives are typically required to be activated as coenzyme A-thioesters prior to their enzyme-catalyzed transfer onto the polyketide synthase. Control over the selection of malonic acid building blocks promises great potential for the experimental alteration of polyketide structure and bioactivity. One requirement for this endeavor is the supplementation of the bacterial polyketide fermentation system with tailored synthetic thioester-activated malonates. The membrane permeable N-acetylcysteamine has been proposed as a coenzyme A-mimic for this purpose. Here, the incorporation efficiency into different polyketides of N-acetylcysteamine activated methylmalonate is studied and quantified, showing a surprisingly high and transferable activity of these polyketide synthase substrate analogues in vivo.
- Subjects :
- Stereochemistry
Coenzyme A
Carboxylic acid
polyketide synthase
Malonic acid
coenzyme A
Cofactor
Full Research Paper
lcsh:QD241-441
Polyketide
chemistry.chemical_compound
Biosynthesis
lcsh:Organic chemistry
polyketide
Polyketide synthase
lcsh:Science
chemistry.chemical_classification
malonic acid
biology
Organic Chemistry
Chemistry
Enzyme
chemistry
biology.protein
lcsh:Q
biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 18605397
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Beilstein Journal of Organic Chemistry
- Accession number :
- edsair.doi.dedup.....28ee247acf357249a3848507391bc6a5