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Simultaneous Single-Molecule Force and Fluorescence Sampling of DNA Nanostructure Conformations Using Magnetic Tweezers
- Source :
- Nano letters. 16(1)
- Publication Year :
- 2015
-
Abstract
- We present a hybrid single-molecule technique combining magnetic tweezers and Forster resonance energy transfer (FRET) measurements. Through applying external forces to a paramagnetic sphere, we induce conformational changes in DNA nanostructures, which are detected in two output channels simultaneously. First, by tracking a magnetic bead with high spatial and temporal resolution, we observe overall DNA length changes along the force axis. Second, the measured FRET efficiency between two fluorescent probes monitors local conformational changes. The synchronized orthogonal readout in different observation channels will facilitate deciphering the complex mechanisms of biomolecular machines.
- Subjects :
- 0301 basic medicine
Magnetic tweezers
Nanostructure
Optical Tweezers
Bioengineering
Tracking (particle physics)
7. Clean energy
Molecular physics
03 medical and health sciences
Paramagnetism
Magnetics
Optics
Fluorescence Resonance Energy Transfer
Nanotechnology
General Materials Science
Fluorescent Dyes
Physics::Biological Physics
Quantitative Biology::Biomolecules
business.industry
Chemistry
Mechanical Engineering
General Chemistry
DNA
equipment and supplies
Condensed Matter Physics
Fluorescence
Nanostructures
030104 developmental biology
Förster resonance energy transfer
Optical tweezers
Temporal resolution
Nucleic Acid Conformation
sense organs
business
Subjects
Details
- ISSN :
- 15306992
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nano letters
- Accession number :
- edsair.doi.dedup.....28fd132f68921e7215ae8810510dc953