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Heterotrophic Foraminifera capable of inorganic nitrogen assimilation
- Source :
- Frontiers in Microbiology, Frontiers in Microbiology, Frontiers Media, 2020, 11, pp.604979. ⟨10.3389/fmicb.2020.604979⟩, Frontiers in Microbiology, Vol 11 (2020), Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2020-12, Vol. 11, P. 604979 (13p.)
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- Nitrogen availability often limits biological productivity in marine systems, where inorganic nitrogen, such as ammonium is assimilated into the food web by bacteria and photoautotrophic eukaryotes. Recently, ammonium assimilation was observed in kleptoplast-containing protists of the phylum foraminifera, possibly via the glutamine synthetase/glutamate synthase (GS/GOGAT) assimilation pathway imported with the kleptoplasts. However, it is not known if the ubiquitous and diverse heterotrophic protists have an innate ability for ammonium assimilation. Using stable isotope incubations (15N-ammonium and 13C-bicarbonate) and combining transmission electron microscopy (TEM) with quantitative nanoscale secondary ion mass spectrometry (NanoSIMS) imaging, we investigated the uptake and assimilation of dissolved inorganic ammonium by two heterotrophic foraminifera; a non-kleptoplastic benthic species, Ammonia sp., and a planktonic species, Globigerina bulloides. These species are heterotrophic and not capable of photosynthesis. Accordingly, they did not assimilate 13C-bicarbonate. However, both species assimilated dissolved 15N-ammonium and incorporated it into organelles of direct importance for ontogenetic growth and development of the cell. These observations demonstrate that at least some heterotrophic protists have an innate cellular mechanism for inorganic ammonium assimilation, highlighting a newly discovered pathway for dissolved inorganic nitrogen (DIN) assimilation within the marine microbial loop.
- Subjects :
- Microbiology (medical)
010504 meteorology & atmospheric sciences
lcsh:QR1-502
Heterotroph
benthic foraminifera
cushman
01 natural sciences
Microbiology
lcsh:Microbiology
03 medical and health sciences
Ammonia
chemistry.chemical_compound
globigerina-bulloides
planktonic-foraminifera
Glutamate synthase
nitrogen cycle
Ammonium
ammonium assimilation
Nitrogen cycle
Original Research
030304 developmental biology
0105 earth and related environmental sciences
0303 health sciences
heterotrophy
denitrification
biology
foraminifera
marine
Assimilation (biology)
Globigerina bulloides
biology.organism_classification
ultrastructure
glutamate-dehydrogenase
heterotrophic protists
chemistry
Environmental chemistry
[SDE]Environmental Sciences
ammonia-tepida
biology.protein
Microbial loop
Subjects
Details
- Language :
- English
- ISSN :
- 1664302X
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology, Frontiers in Microbiology, Frontiers Media, 2020, 11, pp.604979. ⟨10.3389/fmicb.2020.604979⟩, Frontiers in Microbiology, Vol 11 (2020), Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2020-12, Vol. 11, P. 604979 (13p.)
- Accession number :
- edsair.doi.dedup.....2902186c9e8748f581a9fb2b10e8c3b9