Draft genome sequence of a multidrug-resistant CTX-M-15 β-lactamase-producing uropathogenic Escherichia coli isolate (ST131-O25b-H30) from Pakistan exhibiting high potential virulence

Objectives Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTIs). The pandemic sequence type 131 (ST131) clonal group is associated with multidrug resistance and virulence. Here we report the first draft genome of a ST131-O25b- H 30 strain from Pakistan, isolated from a patient with community-acquired UTI. Methods Next-generation sequencing was performed using MiSeq and HiSeq 2500 platforms. De novo assembly of the reads was performed using SPAdes v.3.11. Genomic features were determined with PATRIC and RAST tool kits. Results The 5 327 975-bp draft genome sequence has 5433 coding sequences and 82 tRNAs, an array of antimicrobial resistance genes [ bla CTX-M-15 , bla OXA-1 , bla CMY-2 , sul2 , catB , dfrA17 , mph ( A )], a class 1 integron, 77 insertion sequence (IS) elements, a Tn 3 -like transposon, multiple virulence markers and 7 intact prophage loci. Conclusion In conclusion, the genome sequence of this new UPEC isolate from Pakistan provides a novel insight into the genetic attributes of an epidemic clone associated with a high level of resistance and virulence.