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Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages
- Source :
- Journal of immunology (Baltimore, Md. : 1950). 183(12)
- Publication Year :
- 2009
-
Abstract
- In addition to IL-1 and IL-18, IL-33 was recently identified as a member of the IL-1 cytokine family. rIL-33 can promote production of Th2-type cytokines by Th2 cells and mast cells in vitro. Administration of rIL-33 to mice results in increases in IgE secretion and eosinophilic inflammation. However, the precise immune cell source of IL-33 remains unclear. Moreover, although recombinant pro-IL-33 is cleaved by recombinant caspase-1 in vitro, as are pro-IL-1β and pro-IL-18, the involvement of caspase-1 in pro-IL-33 cleavage remains controversial. In this study, we show that mouse peritoneal macrophages, but not splenic dendritic cells, produced IL-33 upon stimulation with LPS. Likewise, mouse bone marrow cell-derived cultured mast cells also produced a small, but significant amount of IL-33 via FcεRI cross-linking, but not in response to stimulation with LPS. To our surprise, IL-33 release was found even in caspase-1-deficient, caspase-8 inhibitor-treated, and calpain inhibitor-treated macrophages. These observations suggest that caspase-1-, caspase-8-, and calpain-independent IL-33 production by macrophages and/or mast cells may contribute to the pathogenesis of Th2-type allergic inflammation.
- Subjects :
- medicine.medical_treatment
Immunology
Caspase 1
Bone Marrow Cells
Immunoglobulin E
Caspase 8
Allergic inflammation
Mice
Immune system
medicine
Hypersensitivity
Immunology and Allergy
Animals
Mast Cells
Cells, Cultured
Mice, Knockout
Mice, Inbred BALB C
biology
Calpain
Interleukins
Dendritic Cells
Interleukin-33
Cell biology
Interleukin 33
Cytokine
biology.protein
Macrophages, Peritoneal
Inflammation Mediators
Spleen
Subjects
Details
- ISSN :
- 15506606
- Volume :
- 183
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Accession number :
- edsair.doi.dedup.....2916899c4c388f0aa28a69ad518a0041