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Decellularized liver-regenerative 3D printing biomaterials for cell-based liver therapies via a designed procedure combining supercritical fluids with papain-containing reagents
- Source :
- Bio-medical materials and engineering. 33(2)
- Publication Year :
- 2021
-
Abstract
- BACKGROUND: The biologic scaffolds derived from decellularized tissues and organs have been successfully developed in a variety of preclinical and/or clinical studies. OBJECTIVE: The new decellularized liver-regenerative 3D printing biomaterials were designed and prepared for cell-based liver therapies. METHODS: An extraction process was employed to remove the tissue and cellular molecules from porcine liver via pretreatment of supercritical fluid of carbon dioxide (ScCO2). Varying porosities of the decellularized liver tissues were created using papain-containing reagent treatments after ScCO2. RESULTS: The resulting liver-regenerative 3D printing biomaterials of decellularized liver collagen scaffolds were characterized by Fourier transform infrared spectroscopy, thermo-gravimetric analysis, differential scanning calorimetry and scanning electron microscopy. CONCLUSIONS: The decellularized liver collagen scaffolds with good thermal stability (>150 °C) were obtained and employed as liver-regenerative 3D printing biomaterials for cell-based liver therapies.
- Subjects :
- Scaffold
Decellularization
Tissue Engineering
Tissue Scaffolds
Swine
Biomedical Engineering
Biocompatible Materials
General Medicine
Supercritical fluid
Extracellular Matrix
Liver Regeneration
Biomaterials
Papain
chemistry.chemical_compound
chemistry
Liver
Reagent
Porcine liver
Printing, Three-Dimensional
Animals
Indicators and Reagents
Biomedical engineering
Cell based
Subjects
Details
- ISSN :
- 18783619
- Volume :
- 33
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Bio-medical materials and engineering
- Accession number :
- edsair.doi.dedup.....291bcf5f836792168741495a6e2438e4