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ADSC-conditioned media elicit an ex vivo anti-inflammatory macrophage response
- Source :
- Journal of Molecular Endocrinology. 61:173-184
- Publication Year :
- 2018
- Publisher :
- Bioscientifica, 2018.
-
Abstract
- Obesity-associated inflammatory mechanisms play a key role in the pathogenesis of metabolic-related diseases. Failure of anti-inflammatory control mechanisms within adipose tissue and peripheral blood mononuclear cells (PBMCs) have been implicated in disease progression. This study investigated the efficacy of allogeneic adipose tissue-derived mesenchymal stem cells conditioned media (ADSC-CM) to counteract persistent inflammation by inducing an anti-inflammatory phenotype and cytokine response within PBMCs derived from patients with and without metabolic syndrome. Forty-six (n = 46) mixed ancestry females (18–45 years) were subdivided into (a) healthy lean (HL) (n = 10) (BMI 2), (b) overweight/obese (OW/OB) (BMI ≥25 kg/m2, n = 22) and (c) metabolic syndrome (MetS) (visceral adiposity, ≥3 metabolic risk factors) (n = 14) groups. Body composition (DXA scan), metabolic (cholesterol, HDL, LDL, triglycerides, blood glucose) and inflammatory profiles (38-Plex cytokine panel) were determined. PBMCs were isolated from whole blood and treated ex vivo with either (i) autologous participant-derived serum, (ii) ADSCs-CM or (iii) a successive treatment regime. The activation status (CD11b+) and intracellular cytokine (IL6, IL10, TNFa) expression were determined in M1 (CD68+CD206−CD163−) and M2 (CD68+CD163+ CD206+) macrophage populations using flow cytometry. ADSC-CM treatment, promoted a M2 macrophage phenotype and induced IL10 expression, this was most pronounced in the OW/OB group. This response is likely mediated by multiple complementing factors within ADSC-CM, yet to be identified. This study is the first to demonstrate the therapeutic potential of ADSC-CM to restore the inflammatory balance in immune compromised obese individuals.
- Subjects :
- Adult
Blood Glucose
0301 basic medicine
medicine.medical_specialty
Adolescent
medicine.medical_treatment
Adipose tissue
Peripheral blood mononuclear cell
Young Adult
03 medical and health sciences
Endocrinology
Internal medicine
medicine
Humans
Molecular Biology
Cells, Cultured
Inflammation
Tumor Necrosis Factor-alpha
business.industry
Macrophages
Mesenchymal Stem Cells
Middle Aged
Flow Cytometry
medicine.disease
M2 Macrophage
Interleukin 10
030104 developmental biology
Cytokine
Culture Media, Conditioned
Leukocytes, Mononuclear
Female
Metabolic syndrome
business
CD163
Ex vivo
Subjects
Details
- ISSN :
- 14796813 and 09525041
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Endocrinology
- Accession number :
- edsair.doi.dedup.....292864a607a69a27bf3e4cba038145b0