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Yiqi Huoxue Recipe inhibits cardiomyocyte apoptosis caused by heart failure through Keap1/Nrf2/HIF-1α signaling pathway
- Source :
- Bioengineered, article-version (VoR) Version of Record, Bioengineered, Vol 12, Iss 1, Pp 969-978 (2021)
- Publication Year :
- 2021
- Publisher :
- Taylor & Francis, 2021.
-
Abstract
- Yiqi Huoxue Recipe (YHR) is commonly used in China to treat diseases such as heart failure (HF). It has been reported that YHR can treat HF and has a certain protective effect on myocardial cell damage. The purpose of this study is to determine the cardioprotective effects of YHR on HF-induced apoptosis and to clarify its mechanism of action. Oxygen glucose deprivation/recovery (OGD/R) induces H9C2 cell apoptosis model. Ligation of the left anterior descending artery (LAD) coronary artery can induce an animal model of HF. We found that YHR protected H9C2 cells from OGD/R-induced apoptosis, reduced the level of reactive oxygen species (ROS) in H9C2 cells, and increased the mitochondrial membrane potential in H9C2 cells. The results of in vivo animal experiments showed that in the HF model, YHR could reduce infarct area of heart tissue and cardiomyocyte apoptosis rate. YHR regulated the expression of key apoptotic molecules, including increasing the ratio of Bcl-2 and Bax, and reducing the expression of Kelch-like ECH-associated protein 1 (Keap1) and caspase-3. Interestingly, YHR also regulates the expression of NF-E2-related factor 2 (Nrf2) in the nucleus. In summary, YHR may provide cardioprotective effects in heart failure through inhibiting the Keap1/Nrf2/HIF-1α apoptosis pathway.<br />Graphical Abstract
- Subjects :
- 0301 basic medicine
Male
Cell Survival
NF-E2-Related Factor 2
Myocardial Infarction
heart failure
Bioengineering
Apoptosis
030204 cardiovascular system & hematology
Applied Microbiology and Biotechnology
Rats, Sprague-Dawley
03 medical and health sciences
Yiqi Huoxue
0302 clinical medicine
Medicine
Animals
Myocytes, Cardiac
nrf2
keap1
Yiqi Huoxue Recipe (YHR)
Membrane Potential, Mitochondrial
Kelch-Like ECH-Associated Protein 1
business.industry
food and beverages
cardiomyocyte apoptosis
General Medicine
medicine.disease
Hypoxia-Inducible Factor 1, alpha Subunit
KEAP1
Keap1 nrf2
Oxygen
Disease Models, Animal
030104 developmental biology
Glucose
Heart failure
Cancer research
Myocardial cell
hif-1α
Signal transduction
business
Reactive Oxygen Species
Cardiomyocyte apoptosis
TP248.13-248.65
Biotechnology
Research Article
Research Paper
Drugs, Chinese Herbal
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 21655987 and 21655979
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Bioengineered
- Accession number :
- edsair.doi.dedup.....2967e87562ba79a9e67252707fd54837