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From images to interactions: high-resolution phase imaging in tapping-mode atomic force microscopy
- Source :
- Biophysical journal. 80(6)
- Publication Year :
- 2001
-
Abstract
- In tapping-mode atomic force microscopy, the phase shift between excitation and response of the cantilever is used as a material-dependent signal complementary to topography. The localization of information in the phase signal is demonstrated with 1.4-nm lateral resolution on purple membrane of Halobacterium salinarum in buffer solution. In a first-order approximation, the phase signal is found to correlate with modulations of the tip oscillation amplitude, induced by topography. Extending the analysis to contributions of the tip-sample interaction area as a second-order approximation, a method is proposed to extract information about the interaction from the phase signal for surfaces with a roughness in the order of the tip radius.
- Subjects :
- Halobacterium salinarum
Cantilever
business.industry
Chemistry
Phase (waves)
Biophysics
Surface finish
Conductive atomic force microscopy
Radius
Dissipation
Buffers
Microscopy, Atomic Force
Signal
Sensitivity and Specificity
Solutions
Optics
Purple Membrane
Image Processing, Computer-Assisted
Thermodynamics
business
Excitation
Research Article
Subjects
Details
- ISSN :
- 00063495
- Volume :
- 80
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Biophysical journal
- Accession number :
- edsair.doi.dedup.....29d7e4d839d4e22f1de7c3ca17a89185