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Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues
- Source :
- Methods in Molecular Biology ISBN: 9781071608838, Methods in Molecular Biology ISBN: 9781493917075
- Publication Year :
- 2020
- Publisher :
- Springer US, 2020.
-
Abstract
- Immunohistochemical staining is widely used to identify opioid receptors in specific cell types throughout the nervous system. Opioid receptors are not restricted to the central nervous system, but are also present in peripheral sensory neurons, where their activation exerts analgesic effects without inducing centrally mediated side effects. Here, we describe immunohistochemical analysis of μ-opioid receptors in the peripheral sensory neuron cell bodies, along the axons and their peripheral endings in the hind paw skin, as well as in the spinal cord, under naive and sciatic nerve damage conditions in mice. Importantly, we consider the ongoing debate on the specificity of antibodies.
Details
- ISBN :
- 978-1-07-160883-8
978-1-4939-1707-5 - ISBNs :
- 9781071608838 and 9781493917075
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071608838, Methods in Molecular Biology ISBN: 9781493917075
- Accession number :
- edsair.doi.dedup.....29dfe79eb06c67fa65c1b09aa38c6d43