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Regulation of experimental autoimmune uveitis in rats--separation of MHC and non-MHC gene effects
- Publication Year :
- 1991
-
Abstract
- SUMMARYExperimental autoimmune uveitis (EAU) is an organ-specific autoimmune disease and has served as a model of certain ocular inflammatory conditions in man. The present study was aimed at separating the effects of MHC and non-MHC genes on the development of EAU in the rat. EAU-susceptible LEW (RT1l) EAU-resistant WKAH (RTIk), and WKAH.1L (RTF) MHC congenic strain of WKAH background rats were immunized with retinal soluble antigen (S-Ag) in Freund's complete adjuvant (FCA). LEW rats showed typical EAU, while neither WKAH nor WKAH. 1L congenic rats developed EAU. However, when an additional i.v. injection of Bordetella pertussis was given, all rat strains developed EAU. Furthermore, when immunized with peptide M, an 18-mer synthetic peptide, which corresponds to amino acid positions 303–320 of bovine S-Ag, and given an additional i.v. injection of B. pertussis, LEW and WKAH. IL rats developed EAU, whereas WKAH did not. When ACI (RTIavl), BUF (RTIb), LEJ (RTIl), W(RTIk), F344(RTI1v1), BN (RTIq), NIG-III (RTIq), TO (RT1I), and SDJ (RT1u) rats were immunized with peptide M or S-Ag and then given B. pertussis, all strains developed EAU by immunization with S-Ag plus B. pertussis, but only F344 and NIG-III developed EAU by immunization with peptide M. These findings suggest that susceptibility to EAU in rats is controlled by both MHC and non-MHC genes; and that in the absence of B. pertussis adjuvant, the form of disease induced by native S-Ag in FCA is governed by non-MHC gene(s). However, this effect of non-MHC gene(s) could no longer be observed when the rats were also injected with B. pertussis adjuvant at sensitization.
- Subjects :
- Male
Bordetella pertussis
medicine.medical_treatment
Immunology
Genes, MHC Class II
Molecular Sequence Data
Congenic
Genes, MHC Class I
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Major histocompatibility complex
Eye
Autoimmunity
Autoimmune Diseases
Uveitis
Antigen
medicine
Immunology and Allergy
Animals
Hypersensitivity, Delayed
Amino Acid Sequence
Antigens
Eye Proteins
Sensitization
Autoimmune disease
Arrestin
biology
Vaccination
Rats, Inbred Strains
Mycobacterium tuberculosis
medicine.disease
biology.organism_classification
eye diseases
Peptide Fragments
Rats
medicine.anatomical_structure
biology.protein
sense organs
Disease Susceptibility
Adjuvant
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....2a00ef26f5db6cf37062fe47ca537446