Back to Search
Start Over
Exosomal <scp>LINC01005</scp> derived from oxidized low‐density lipoprotein‐treated endothelial cells regulates vascular smooth muscle cell phenotypic switch
- Source :
- BioFactors. 46:743-753
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Phenotype switch of vascular smooth muscle cells (VSMCs) plays an important role in the development of atherosclerosis (AS). Endothelial cells can regulate VSMC phenotypic switch by secreting exosomes, crucial mediators of intracellular communication. This study aimed to determine whether exosomal LINC01005 from oxidized low-density lipoprotein (ox-LDL)-treated human umbilical vein endothelial cells (HUVECs) plays a role in regulating VSMC phenotypic switch and to validate the underlying molecular mechanism. Exosomes were extracted from ox-LDL-treated HUVECs (ox-LDL-Exo) and then administered into VSMCs. VSMC phenotypic switch was assessed by determining VSMC phenotypic markers using western blot. VSMC cell proliferation and migration were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and wound healing assay, respectively. The interaction between miR-128-3p and LINC01005 or Krüppel-like factor 4 (KLF4) was analyzed by luciferase reporter assay. ox-LDL-Exo contained high expression of LINC01005. Inhibition of LINC01005 expression in ox-LDL-Exo abrogated the ox-LDL-Exo-induced VSMC phenotypic switch, proliferation, and migration. Furthermore, LINC01005 acted as a sponge of miR-128-3p to upregulate KLF4 expression. Moreover, miR-128-3p overexpression and KLF4 silencing in VSMCs attenuated the ox-LDL-Exo-induced VSMC phenotypic switch, proliferation, and migration. Collectively, exosomal LINC01005 from ox-LDL-treated HUVECs promotes VSMC phenotype switch, proliferation, and migration by regulating the miR-128-3p/KLF4 axis.
- Subjects :
- 0301 basic medicine
Vascular smooth muscle
Myocytes, Smooth Muscle
Clinical Biochemistry
Cell
Kruppel-Like Transcription Factors
Exosomes
Biochemistry
Muscle, Smooth, Vascular
Umbilical vein
Kruppel-Like Factor 4
03 medical and health sciences
0302 clinical medicine
Western blot
Downregulation and upregulation
Cell Movement
Human Umbilical Vein Endothelial Cells
medicine
Humans
Cell Proliferation
medicine.diagnostic_test
Chemistry
Cell growth
Endothelial Cells
General Medicine
musculoskeletal system
Microvesicles
Cell biology
Lipoproteins, LDL
MicroRNAs
Phenotype
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
KLF4
030220 oncology & carcinogenesis
cardiovascular system
Molecular Medicine
RNA, Long Noncoding
Subjects
Details
- ISSN :
- 18728081 and 09516433
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- BioFactors
- Accession number :
- edsair.doi.dedup.....2a17c2f347e5c75a2c6f24384ddbb8e5