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An Ex-Vivo Culture System of Ovarian Cancer Faithfully Recapitulating the Pathological Features of Primary Tumors

Authors :
Kenji Yamada
Kasumi Dendo
Masayuki Yoshida
Reiko Watanabe
Katsuyuki Tanaka
Mitsuya Ishikawa
Yuki Yoshimatsu
Naoki Goshima
Farhana Ishrat Ghani
Tomomi Nakahara
Takashi Yugawa
Hiroshi Yoshida
Tomoyasu Kato
Tohru Kiyono
Source :
Cells, Vol 8, Iss 7, p 644 (2019), Cells, Volume 8, Issue 7
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

The success rate of establishing human cancer cell lines is not satisfactory and the established cell lines often do not preserve the molecular and histological features of the original tissues. In this study, we developed a novel culture method which can support proliferation of almost all primary epithelial ovarian cancer cells, as well as primary normal human oviductal epithelial cells. Cancer cells from fresh or frozen specimens were enriched by the anti-EpCAM antibody-conjugated magnetic beads, plated on Matrigel-coated plate and cultivated under the optimized culture conditions. Seventeen newly established ovarian cancer cell lines, which included all four major histotypes of ovarian cancer, were confirmed to express histotype-specific markers in vitro. Some of the cell lines from all the four histotypes, except mucinous type, generated tumors in immune-deficient mice and the xenograft tumor tissues recapitulated the corresponding original tissues faithfully. Furthermore, with poorly tumorigenic cell lines including mucinous type, we developed a novel xenograft model which could reconstruct the original tissue architecture through forced expression of a set of oncogenes followed by its silencing. With combination of the novel culture method and cell-derived xenograft system, virtually every epithelial ovarian cancer can be reconstituted in mice in a timely fashion.

Details

Language :
English
ISSN :
20734409
Volume :
8
Issue :
7
Database :
OpenAIRE
Journal :
Cells
Accession number :
edsair.doi.dedup.....2a21861caa3df799a7dde329c6047591