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Comparison of High-Performance Liquid Chromatography and Monoclonal Fluorescence Polarization Immunoassay for the Determination of Whole-Blood Cyclosporin A in Liver and Heart Transplant Patients
- Source :
- Therapeutic Drug Monitoring. 16:526-530
- Publication Year :
- 1994
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1994.
-
Abstract
- Conflicting conclusions have been drawn from comparisons of high-performance liquid chromatography (HPLC) and the Abbott Diagnostics monoclonal fluorescence polarization immunoassay (mFPIA) for cyclosporin. The aim of this study was to compare whole blood cyclosporin A (CsA) concentrations measured by both mFPIA and HPLC in liver and heart transplant patients. One hundred and twenty-four liver and 62 heart transplant patient samples were assayed by both methods. Assay imprecision for both methods during the studies was < 7% over the range 150-800 micrograms/L. At an HPLC-determined concentration of 100 micrograms/L, mFPIA overestimated CsA by 60% (liver) and 77% (heart). At 300 micrograms/L, the overestimation was 40% (liver) and 45% (heart). On this basis, the mFPIA is not interchangeable with HPLC.
- Subjects :
- Pharmacology
Chromatography
Heart disease
Chemistry
Antibodies, Monoclonal
medicine.disease
High-performance liquid chromatography
Liver Transplantation
Transplantation
Cyclosporin a
Calibration
Fluorescence Polarization Immunoassay
Monoclonal
Cyclosporine
medicine
Fluorescence polarization immunoassay
Heart Transplantation
Humans
Pharmacology (medical)
Reagent Kits, Diagnostic
Quantitative analysis (chemistry)
Chromatography, High Pressure Liquid
Whole blood
Subjects
Details
- ISSN :
- 01634356
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Therapeutic Drug Monitoring
- Accession number :
- edsair.doi.dedup.....2a9b54c447519f78022320c802e13ecf