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Additional file 1 of First time β-farnesene production by the versatile bacterium Cupriavidus necator
- Publication Year :
- 2021
- Publisher :
- figshare, 2021.
-
Abstract
- Additional file 1. The construction of the plasmids to produce farnesene is described in detail. In addition, the MVA pathway (plasmid pKRTK3) coupled with the β-farnesene synthase (plasmid pBBR1c_farn) expression is presented. The calculations of the oxygen and carbon dioxide transfer rates (OTR/CTR) via the gas-balancing method is shown. Furthermore, the β-farnesene calibration with caffeine as internal standard is described.
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....2ac0bce5342bbcea747a5b1a7132c3c3
- Full Text :
- https://doi.org/10.6084/m9.figshare.14489896.v1