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Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysisviasuppression of sirtuin 3 in pancreatic cancer
- Source :
- World Journal of Gastroenterology
- Publication Year :
- 2018
- Publisher :
- Baishideng Publishing Group Inc., 2018.
-
Abstract
- AIM To uncover the roles of tumor-promoting gene ZEB1 in aerobic glycolysis regulation and shed light on the underlying molecular mechanism. METHODS Endogenous zinc finger E-box binding homeobox-1 (ZEB1) was silenced using a lentivirus-mediated method, and the impact of ZEB1 and methyl-CpG binding domain protein 1 (MBD1) on aerobic glycolysis was measured using seahorse cellular flux analyzers, reactive oxygen species quantification, and mitochondrial membrane potential measurement. The interaction between ZEB1 and MBD1 was assessed by co-immunoprecipitation and immunofluorescence assays. The impact of ZEB1 and MBD1 interaction on sirtuin 3 (SIRT3) expression was confirmed by quantitative polymerase chain reaction, western blotting, and dual-luciferase and chromatin-immunoprecipitation assays. RESULTS ZEB1 was a positive regulator of aerobic glycolysis in pancreatic cancer. ZEB1 transcriptionally silenced expression of SIRT3, a mitochondrial-localized tumor suppressor, through interaction with MBD1. CONCLUSION ZEB1 silenced SIRT3 expression via interaction with MBD1 to promote aerobic glycolysis in pancreatic cancer.
- Subjects :
- 0301 basic medicine
Methyl-CpG binding domain protein 1
03 medical and health sciences
0302 clinical medicine
Sirtuin 3
Cell Line, Tumor
Pancreatic cancer
medicine
Humans
Glycolysis
Zinc finger
Zinc finger E-box binding homeobox-1
biology
Chemistry
Tumor Suppressor Proteins
Gastroenterology
Zinc Finger E-box-Binding Homeobox 1
General Medicine
Basic Study
medicine.disease
Mitochondria
Cell biology
DNA-Binding Proteins
Gene Expression Regulation, Neoplastic
Pancreatic Neoplasms
030104 developmental biology
Anaerobic glycolysis
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Sirtuin
biology.protein
Homeobox
Transcription Factors
Subjects
Details
- ISSN :
- 10079327
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- World Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....2acaa4ce00abfcef5cf7ed3946835d39